ArticleScientific reports2019
Palindromic sequence-targeted (PST) PCR: a rapid and efficient method for high-throughput gene characterization and genome walking.
Article in Scientific reports, 2019. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 20 papers.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
20 citing papers in PubMed.
- Transgene Mapping in Animals: What to Choose?International journal of molecular sciences · 2025Review
- Protocol to Identify Unknown Flanking DNA Using Partially Overlapping Primer-based PCR for Genome Walking.Bio-protocol · 2025Article
- An Assay for Variable Fragment Length Allele-Specific Genotyping.Methods in molecular biology (Clifton, N.J.) · 2025Article
- Arbitrarily Suffixed Sequence-Specific Primer PCR for Reliable Genome Walking: Taking Genome Walkings ofIranian journal of biotechnology · 2024Article
- Genetic Diversity in Natural Populations ofGenes · 2023Article
- Transposable elements: multifunctional players in the plant genome.Frontiers in plant science · 2023Review
- High-throughput detection of T-DNA insertion sites for multiple transgenes in complex genomes.BMC genomics · 2022Article
- Editorial: Innovative applications of sequencing technologies in plant science.Frontiers in plant science · 2022Article
- A Guide to Using FASTPCR Software for PCR, In Silico PCR, and Oligonucleotide Analysis.Methods in molecular biology (Clifton, N.J.) · 2022Article
- Primer Binding Site (PBS) Profiling of Genetic Diversity of Natural Populations of Endemic SpeciesBiotech (Basel (Switzerland)) · 2021Article
- Proof of principle for piggyBac-mediated transgenesis in the flatworm Macrostomum lignano.Genetics · 2021Article
- Amplicon genome fishing (AGF): a rapid and efficient method for sequencing target cis-regulatory regions in nonmodel organisms.Molecular genetics and genomics : MGG · 2021Article
- Genetic Diversity and Pathogenicity ofJournal of fungi (Basel, Switzerland) · 2021Article
- Isolation and Purification of DNA from Complicated Biological Samples.Methods in molecular biology (Clifton, N.J.) · 2021Article
- Article
- Palindromic Sequence-Targeted (PST) PCR, Version 2: An Advanced Method for High-Throughput Targeted Gene Characterization and Transposon Display.Frontiers in plant science · 2021Article
- Retrotransposable Elements: DNA Fingerprinting and the Assessment of Genetic Diversity.Methods in molecular biology (Clifton, N.J.) · 2021Article
- Simple innovative adaptor to improve genome walking with convenient PCR.Journal, genetic engineering & biotechnology · 2020Article
- Long Tandem Arrays ofInternational journal of molecular sciences · 2020Article
- Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
5 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Genome walking (GW) refers to the capture and sequencing of unknown regions in a long DNA molecule that are adjacent to a region with a known sequence. A novel PCR-based method, palindromic sequence-targeted PCR (PST-PCR), was developed. PST-PCR is based on a distinctive design of walking primers and special thermal cycling conditions. The walking primers (PST primers) match palindromic sequences (PST sites) that are randomly distributed in natural DNA. The PST primers have palindromic sequences at their 3'-ends. Upstream of the palindromes there is a degenerate sequence (8-12 nucleotides long); defined adapters are present at the 5'-termini. The thermal cycling profile has a linear amplification phase and an exponential amplification phase differing in annealing temperature. Changing the annealing temperature to switch the amplification phases at a defined cycle controls the balance between sensitivity and specificity. In contrast to traditional genome walking methods, PST-PCR is rapid (two to three hours to produce GW fragments) as it uses only one or two PCR rounds. Using PST-PCR, previously unknown regions (the promoter and intron 1) of the VRN1 gene of Timothy-grass (Phleum pratense L.) were captured for sequencing. In our experience, PST-PCR had higher throughput and greater convenience in comparison to other GW methods.
Indexed as
Identifiers
What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.