Evidence map›Paper›PMID 31775775›Full record

ArticleBMC biotechnology2019

Engineering integrative vectors based on phage site-specific recombination mechanism for Lactococcus lactis.

Innanurdiani Koko, Adelene Ai-Lian Song, Mas Jaffri Masarudin, Raha Abdul Rahim

Abstract read
In one paragraph

Article in BMC biotechnology, 2019. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 4 papers.

0numbers the graph read from it
0cells of the map it votes in
4citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

4 citing papers in PubMed.

  1. Review
  2. Article
  3. Review
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4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

4 authors.

Innanurdiani KokoDepartment of Cell and Molecular Biology, Faculty of Biotechnology and Biomolecular Sciences, University Putra Malaysia, 43400 UPM, Serdang, Selangor, Malaysia.
Adelene Ai-Lian SongDepartment of Microbiology, Faculty of Biotechnology and Biomolecular Sciences, University Putra Malaysia, 43400 UPM, Serdang, Selangor, Malaysia. adelene@upm.edu.my.ORCID 0000-0002-5413-3314
Mas Jaffri MasarudinDepartment of Cell and Molecular Biology, Faculty of Biotechnology and Biomolecular Sciences, University Putra Malaysia, 43400 UPM, Serdang, Selangor, Malaysia.
Raha Abdul RahimDepartment of Cell and Molecular Biology, Faculty of Biotechnology and Biomolecular Sciences, University Putra Malaysia, 43400 UPM, Serdang, Selangor, Malaysia. raha@upm.edu.my.

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

backgroundSite-specific integration system allows foreign DNA to be integrated into the specific site of the host genome, enabling stable expression of heterologous protein. In this study, integrative vectors for secretion and surface display of proteins were constructed based on a lactococcal phage TP901-1 integrating system.

resultsThe constructed integration system comprises of a lactococcal promoter (P

conclusionThe engineered synthetic integrative vectors have the potential to be used for secretion or surface display of heterologous protein production in lactococcal expression system for research or industrial purposes, especially in live vaccine delivery.

Indexed as

Recombination, GeneticAttachment Sites, MicrobiologicalBacteriophagesGenetic EngineeringGenetic VectorsGenome, BacterialLactococcus lactisProtein Sorting SignalsVirus IntegrationProtein Sorting SignalsIntegrative vectorLactococcus lactisSecretionSignal peptideSurface display

Identifiers

PMID31775775
PMCPMC6882331

What OpenQuestion holds

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Registered trials

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.