ArticleBMC biology2019
Optimized photo-stimulation of halorhodopsin for long-term neuronal inhibition.
Article in BMC biology, 2019. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 24 papers.
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Who cites it
24 citing papers in PubMed, 42 citations in OpenAlex.
- Optogenetics meets automated patch clamp.iScience · 2026Article
- X-optogenetic inhibition of VTA GABA neurons for wireless deep-brain stimulation and suppression of scratching behavior in freely moving mice.Materials today. Bio · 2026Article
- A Novel Long-range Excitatory Neural Circuit from the Magnocellular Red Nucleus to Spinal Dorsal Horn Facilitates Neuropathic Pain-like Behaviors in Male Mice.Neuroscience bulletin · 2026Article
- Single-Die-Level MEMS Post-Processing for Prototyping CMOS-Based Neural Probes Combined with Optical Fibers for Optogenetic Neuromodulation.Micromachines · 2026Article
- Stabilized Ion Selectivity Corrects Activation Drift in Kalium Channelrhodopsins.Advanced science (Weinheim, Baden-Wurttemberg, Germany) · 2026Article
- 3D-printed optogenetic neural probe integrated with microfluidic tube for opsin/drug delivery.Scientific reports · 2025Article
- Article
- Ultra-Low-Dose UV-C Photo-stimulation Promotes Neural Stem Cells Differentiation via Presenilin 1 Mediated Notch and β-Catenin Activation.Molecular neurobiology · 2024Article
- Optogenetic β cell interrogation in vivo reveals a functional hierarchy directing the CaScience advances · 2024Article
- Optogenetic Inhibition of Rat Anterior Cingulate Cortex Impairs the Ability to Initiate and Stay on Task.The Journal of neuroscience : the official journal of the Society for Neuroscience · 2024Article
- Electroacupuncture pretreatment mediates sympathetic nerves to alleviate myocardial ischemia-reperfusion injury via CRH neurons in the paraventricular nucleus of the hypothalamus.Chinese medicine · 2024Article
- Effects of optogenetic silencing the anterior cingulate cortex in a delayed non-match to trajectory task.Oxford open neuroscience · 2024Article
- Review
- Review
- Network instability dynamics drive a transient bursting period in the developing hippocampus in vivo.eLife · 2022Article
- Thalamic bursting and the role of timing and synchrony in thalamocortical signaling in the awake mouse.Neuron · 2022Article
- Article
- Striatal DFrontiers in aging neuroscience · 2022Article
- Advances and prospects of rhodopsin-based optogenetics in plant research.Plant physiology · 2021Review
- A limited role of NKCC1 in telencephalic glutamatergic neurons for developing hippocampal network dynamics and behavior.Proceedings of the National Academy of Sciences of the United States of America · 2021Article
Corrections and comments
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Authors and funding
8 authors at 2 institutions in 3 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
backgroundOptogenetic silencing techniques have expanded the causal understanding of the functions of diverse neuronal cell types in both the healthy and diseased brain. A widely used inhibitory optogenetic actuator is eNpHR3.0, an improved version of the light-driven chloride pump halorhodopsin derived from Natronomonas pharaonis. A major drawback of eNpHR3.0 is related to its pronounced inactivation on a time-scale of seconds, which renders it unsuited for applications that require long-lasting silencing.
resultsUsing transgenic mice and Xenopus laevis oocytes expressing an eNpHR3.0-EYFP fusion protein, we here report optimized photo-stimulation techniques that profoundly increase the stability of eNpHR3.0-mediated currents during long-term photo-stimulation. We demonstrate that optimized photo-stimulation enables prolonged hyperpolarization and suppression of action potential discharge on a time-scale of minutes.
conclusionsCollectively, our findings extend the utility of eNpHR3.0 to the long-lasting inhibition of excitable cells, thus facilitating the optogenetic dissection of neural circuits.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.