ArticleBMC plant biology2019
Polysaccharide biosynthetic pathway profiling and putative gene mining of Dendrobium moniliforme using RNA-Seq in different tissues.
Article in BMC plant biology, 2019. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 18 papers.
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18 citing papers in PubMed, 40 citations in OpenAlex.
- Transcriptomic and Metabolomic Insights into Tissue- and Cultivar-Dependent Polysaccharide Accumulation inInternational journal of molecular sciences · 2026Article
- The transcriptomic and metabolomic mechanisms responsible for high medicinal quality in Dendrobium huoshanense under different cultivation techniques.BMC plant biology · 2026Article
- Deciphering the Biosynthetic Pathways and Regulatory Networks of the Active Components ofInternational journal of molecular sciences · 2026Article
- Exploring Fungal Communication Mechanisms in the Rhizosphere Microbiome for a Sustainable Green Agriculture.Journal of fungi (Basel, Switzerland) · 2025Review
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- A chromosome-levelActa pharmaceutica Sinica. B · 2025Article
- Diversity of organ-specific plant transcriptomes.Acta biochimica Polonica · 2025Review
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- Multi-omics profiling reveal responses of three majorFrontiers in plant science · 2024Article
- Transcriptome analysis of three medicinal plants of the genusFrontiers in plant science · 2023Article
- A Comparison of the Flavonoid Biosynthesis Mechanisms ofInternational journal of molecular sciences · 2022Article
- Transcriptome Analysis of Different Sections of Rhizome in Polygonatum sibiricum Red. and Mining Putative Genes Participate in Polysaccharide Biosynthesis.Biochemical genetics · 2022Article
- Genome-wide association studies reveal putative QTLs for physiological traits under contrasting phosphorous conditions in wheat (Frontiers in genetics · 2022Article
- Orchid Phylotranscriptomics: The Prospects of Repurposing Multi-Tissue Transcriptomes for Phylogenetic Analysis and Beyond.Frontiers in plant science · 2022Article
- Article
- A candidate gene identified in converting platycoside E to platycodin D from Platycodon grandiflorus by transcriptome and main metabolites analysis.Scientific reports · 2021Article
- Composition and diversity of bacterial communities in the rhizosphere of the Chinese medicinal herb Dendrobium.BMC plant biology · 2021Article
- Comparative Transcriptome Analysis of DifferentInternational journal of molecular sciences · 2020Article
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Authors and funding
6 authors at 2 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
backgroundDendrobium moniliforme (Linnaeus) Swartz is a well-known plant used in traditional Chinese medicine due to bioactive constituents. Polysaccharides are the main medicinal ingredients, yet no studies have been published on polysaccharide biosynthesis in D. moniliforme. To comprehensively investigate the polysaccharide at the transcription level, we performed de novo transcriptome sequencing for the first time to produce a comprehensive transcriptome of D. moniliforme.
resultsIn our study, a database of 562,580 unigenes (average length = 1115.67 bases) was generated by performing transcriptome sequencing. Based on the gene annotation of the transcriptome, we identified 1204 carbohydrate-active related unigenes against CAZy database, including 417 glycosyltransferase genes (GTs), 780 glycoside hydrolases (GHs), 19 carbohydrate esterases (CEs), 75 carbohydrate-binding modules (CBMs), and 44 polysaccharide lyases (PLs). In the cellulose synthase family, 21 differential expression genes (DEGs) related to polysaccharide were identified. Subsequently, the tissue-specific expression patterns of the genes involved in polysaccharide pathway were investigated, which provide understanding of the biosynthesis and regulation of DMP at the molecular level. The two key enzyme genes (Susy and SPS) involved in the polysaccharide pathway were identified, and their expression patterns in different tissues were further analyzed using quantitative real-time PCR.
conclusionsWe determined the content of polysaccharides from Dendrobium moniliforme under different tissues, and we obtained a large number of differential genes by transcriptome sequencing. This database provides a pool of candidate genes involved in biosynthesis of polysaccharides in D. moniliforme. Furthermore, the comprehensive analysis and characterization of the significant pathways are expected to give a better insight regarding the diversity of chemical composition, synthetic characteristics, and the regulatory mechanism which operate in this medical herb.
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