ArticleBMC genomics2019
DNA methylation patterns vary in boar sperm cells with different levels of DNA fragmentation.
Article in BMC genomics, 2019. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 22 papers.
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Who cites it
22 citing papers in PubMed.
- Preliminary study of pregnancy rates and litter sizes following artificial insemination of boar spermatozoa prepared by colloid centrifugation and hypothermic storage.Scientific reports · 2025Article
- Comprehensive multi-omics analysis uncovers potential risks of aged sperm on offspring development after short-term storage.BMC biology · 2025Article
- Sperm protein profiles and their correlation with DNA integrity and protamine deficiency in Donggala bullsVeterinary world · 2025Article
- Effect of Colloid Centrifugation and Cold Storage of Boar Semen at 4 °C on Bacterial Load and Sperm Quality.Antibiotics (Basel, Switzerland) · 2025Article
- Prediction of methylation status using WGS data of plasma cfDNA for multi-cancer early detection (MCED).Clinical epigenetics · 2024Article
- Sperm quality andFrontiers in veterinary science · 2024Article
- Exploring the full potential of sperm function with nanotechnology tools.Animal reproduction · 2024Article
- A DNA Methylation Perspective on Infertility.Genes · 2023Review
- Sperm Chromatin Status and DNA Fragmentation in Mouse Species with Divergent Mating Systems.International journal of molecular sciences · 2023Article
- Sperm quality parameters, fertilizing potential, metabolites, and DNA methylation in cold-stored and cryopreserved milt from Atlantic salmon (Frontiers in genetics · 2023Article
- Article
- Sperm DNA methylation patterns at discrete CpGs and genes involved in embryonic development are related to bull fertility.BMC genomics · 2022Article
- How Epigenetics Can Enhance Pig Welfare?Animals : an open access journal from MDPI · 2021Review
- The potential of sperm bovine protamine as a protein marker of semen production and quality at the National Artificial Insemination Center of Indonesia.Veterinary world · 2021Article
- Sperm DNA integrity status is associated with DNA methylation signatures of imprinted genes and non-imprinted genes.Journal of assisted reproduction and genetics · 2021Article
- Sperm Methylome Profiling Can Discern Fertility Levels in the Porcine Biomedical Model.International journal of molecular sciences · 2021Article
- Effects of Environmental and Pathological Hypoxia on Male Fertility.Frontiers in cell and developmental biology · 2021Review
- Global Methylation and Protamine Deficiency in Ram Spermatozoa Correlate with Sperm Production and Quality but Are Not Influenced by Melatonin or Season.Animals : an open access journal from MDPI · 2020Article
- Unraveling epigenomic abnormality in azoospermic human males by WGBS, RNA-Seq, and transcriptome profiling analyses.Journal of assisted reproduction and genetics · 2020Article
- Sperm DNA Hypomethylation Proximal to Reproduction Pathway Genes in Maturing Elite Norwegian Red Bulls.Frontiers in genetics · 2020Article
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11 authors.
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Abstract
backgroundSperm DNA integrity is considered essential for successful transmission of the paternal genome, fertilization and normal embryo development. DNA fragmentation index (DFI, %) has become a key parameter in the swine artificial insemination industry to assess sperm DNA integrity. Recently, in some elite Norwegian Landrace boars (boars with excellent field fertility records), a higher level of sperm DFI has been observed. In order to obtain a better understanding of this, and to study the complexity of sperm DNA integrity, liquid preserved semen samples from elite boars with contrasting DFI levels were examined for protamine deficiency, thiol profile and disulphide bonds. Additionally, the DNA methylation profiles of the samples were determined by reduced representation bisulphite sequencing (RRBS).
resultsIn this study, different traits related to sperm DNA integrity were investigated (n = 18 ejaculates). Upon liquid storage, the levels of total thiols and disulphide bonds decreased significantly, while the DFI and protamine deficiency level increased significantly. The RRBS results revealed similar global patterns of low methylation from semen samples with different levels of DFI (low, medium and high). Differential methylation analyses indicated that the number of differentially methylated cytosines (DMCs) increased in the low-high compared to the low-medium and the medium-high DFI groups. Annotating the DMCs with gene and CpG features revealed clear differences between DFI groups. In addition, the number of annotated transcription starting sites (TSS) and associated pathways in the low-high comparison was greater than the other two groups. Pathway analysis showed that genes (based on the closest TSS to DMCs) corresponding to low-high DFI comparison were associated with important processes such as membrane function, metabolic cascade and antioxidant defence system.
conclusionTo our knowledge, this is the first study evaluating DNA methylation in boar sperm cells with different levels of DFI. The present study shows that sperm cells with varying levels of DNA fragmentation exhibit similar global methylation, but different site-specific DNA methylation signatures. Moreover, with increasing DNA fragmentation in spermatozoa, there is an increase in the number of potentially affected downstream genes and their respective regulatory pathways.
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