ArticleCells2019
A New Sensitive Method for the Detection of Mycoplasmas Using Fluorescence Microscopy.
Article in Cells, 2019. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 20 papers.
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Who cites it
20 citing papers in PubMed, 27 citations in OpenAlex.
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- Synthesis and characterization of NIR-sensitive curcumin-gelatin nanoparticles for targeted drug delivery in 3D colon cancer.Scientific reports · 2026Article
- Production of small-scale laboratory-grown cell-based fish meat from Asian seabass muscle and fin cell lines.In vitro cellular & developmental biology. Animal · 2026Article
- Evaluation of multiple methods for the diagnosis of equine Hemomycoplasmosis in Misan, Iraq.Open veterinary journal · 2026Article
- Capsular polysaccharide of Mycoplasma mycoides subsp. capri contributes to phenotypic diversity promoting distinctive immune responses.PLoS pathogens · 2025Article
- Article
- Establishment and Characterization of Novel Channa striata Muscle Cell Line for Scaffold-Based Micro-tissue Production.Marine biotechnology (New York, N.Y.) · 2025Article
- A Rapid Approach for Identifying Cell Lines Lacking Functional Cytidine Deaminase.International journal of molecular sciences · 2025Article
- The kinetics of uracil-N-glycosylase distribution inside replication foci.Scientific reports · 2025Article
- Development and characterization of primary cell culture from the spinal cord of Asian seabass, Lates calcarifer.In vitro cellular & developmental biology. Animal · 2024Article
- A new technique for the analysis of metabolic pathways of cytidine analogues and cytidine deaminase activities in cells.Scientific reports · 2023Article
- A Systematic Review of the Recent Techniques Commonly Used in the Diagnosis ofPathogens (Basel, Switzerland) · 2023Review
- The effects of Avemar treatment on feline immunodeficiency virus infected cell cultures.Veterinary medicine and science · 2023Article
- MEK Is a Potential Indirect Target in Subtypes of Head and Neck Cancers.International journal of molecular sciences · 2023Article
- Chrysophanol Suppresses Cell Growth via mTOR/PPAR-α Regulation and ROS Accumulation in Cultured Human Tongue Squamous Carcinoma SAS Cells.Current issues in molecular biology · 2022Article
- Anchored linear oligonucleotides: the effective tool for the real-time measurement of uracil DNA glycosylase activity.Open biology · 2021Article
- New Concept and Apparatus for Cytocentrifugation and Cell Processing for Microscopy Analysis.International journal of molecular sciences · 2021Article
- High ATF4 Expression Is Associated With Poor Prognosis, Amino Acid Metabolism, and Autophagy in Gastric Cancer.Frontiers in oncology · 2021Article
- Aptamer Cocktail to Detect Multiple Species of Mycoplasma in Cell Culture.International journal of molecular sciences · 2020Article
Corrections and comments
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Authors and funding
7 authors at 3 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Contamination of cell cultures by mycoplasmas is a very common phenomenon. As they can substantially alter cell metabolism and potentially spread to all cell cultures in laboratory, their early detection is necessary. One of the fastest and cheapest methods of mycoplasma detection relies on the direct staining of mycoplasmas' DNA by DAPI or Hoechst dyes. Although this method is easy and fast to perform, it suffers from the low signal provided by these dyes compared to the nuclear DNA. Therefore, the reporter cell lines are used for cultivation of mycoplasmas before DAPI or the Hoechst staining step. In the study presented, we have developed and tested a new immunofluorescence assay for the detection of mycoplasmas. The method is based on the enzymatic labeling using DNA polymerase I and modified nucleotides utilizing nicks in the mycoplasmas' DNA. Modified nucleotides are incorporated into mycoplasmas' DNA and subsequently visualized by immunofluorescence microscopy. The developed approach is independent of the mycoplasma strain, does not intensely stain nuclear DNA, does not stain other bacteria, and provides higher sensitivity than the approach based on the direct labeling using DAPI or Hoechst dyes.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.