Evidence map›Paper›PMID 31773572›Full record

ArticleMethods in molecular biology (Clifton, N.J.)2020

Metabolic Labeling of Inositol Phosphates and Phosphatidylinositols in Yeast and Mammalian Cells.

Andrew T Hale, Bradley P Clarke, John D York

Open access · greenAbstract read
In one paragraph

Article in Methods in molecular biology (Clifton, N.J.), 2020. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 4 papers.

0numbers the graph read from it
0cells of the map it votes in
4citing papers in PubMed
1.5field-weighted citation impact, top 16% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

4 citing papers in PubMed, 8 citations in OpenAlex.

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4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

3 authors at 1 institution in 1 country.

Andrew T HaleDepartment of Biochemistry, Vanderbilt University School of Medicine, Nashville, TN, USA.
Bradley P ClarkeDepartment of Biochemistry, Vanderbilt University School of Medicine, Nashville, TN, USA.
John D YorkDepartment of Biochemistry, Vanderbilt University School of Medicine, Nashville, TN, USA. John.york@vanderbilt.edu.
Vanderbilt University · US

Funding

MEDICAL SCIENTIST TRAINING PROGRAMT32GM007347 · NIGMS · VANDERBILT UNIVERSITY · PI WILLIAMS, CHRISTOPHER S. · 1985 to 2023
$26.3M
The Role of Inositol Signaling in Human DiseaseR01HL055672 · NHLBI · WASHINGTON UNIVERSITY · PI YORK, JOHN D. · 1996 to 2012
$5.4M
Medical Scientist Training ProgramT32GM152284 · NIGMS · VANDERBILT UNIVERSITY · PI Christopher S. Williams · 2024 to 2026
$4.8M
Inositol hexakisphosphate regulation of N-terminal acetyltransferasesR01GM124404 · NIGMS · VANDERBILT UNIVERSITY · PI YORK, JOHN D. · 2017 to 2020
$1.6M
NHLBI NIH HHS R01 HL055672NIGMS NIH HHS R01 GM124404NIGMS NIH HHS T32 GM007347NIGMS NIH HHS T32 GM152284
6 · The paper itself

Abstract

Inositol phosphate (IP) and phosphatidylinositol (PI) signaling are critical signal transduction pathways responsible for generating numerous receptor-mediated cellular responses. Biochemical and genetic studies have revealed diverse roles of IP and PI signaling in eukaryotic signaling, but detailed characterization of unique IP and PI signaling profiles in response to different agonists and among cell types remains largely unexplored. Here, we outline steady-state inositol metabolic-labeling techniques that can be leveraged to assess the IP and PI signaling state in eukaryotic cells. This flexible technique can be amended and optimized to your cell line of interest, perturbed with biochemical, genetic, or pharmacological alteration, and used to provide comprehensive inositol profiling in various cellular systems.

Indexed as

AnimalsBiochemical PhenomenaCell LineChlorocebus aethiopsCOS CellsHeLa CellsHT29 CellsHumansInositol PhosphatesPhosphatidylinositolsSaccharomyces cerevisiaeSignal TransductionInositol PhosphatesPhosphatidylinositolsHPLCInositol phosphatesLipid signalingMetabolic labelingPhosphatidylinositolSignal transduction

Identifiers

PMID31773572
PMCPMC7293352
OpenAlexW2991087272

What OpenQuestion holds

Textmetadata
LicenceTDM
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.