Evidence map›Paper›PMID 31773316›Full record

ArticleInternational journal of legal medicine2020

Postmortem interval determination using mRNA markers and DNA normalization.

Duo Peng, Meili Lv, Zhilong Li, Huan Tian, ShengQiu Qu, Bo Jin, Bing Long, Weibo Liang, Lin Zhang

Abstract read
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In one paragraph

Article in International journal of legal medicine, 2020. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 20 papers, 1 of them a synthesis that pooled it.

0numbers the graph read from it
0cells of the map it votes in
20citing papers in PubMed, 1 pooled it
0.9field-weighted citation impact, top 25% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

20 citing papers in PubMed, 1 synthesis or guideline pooled it, 33 citations in OpenAlex.

  1. Pooled it
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  3. Review
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  6. RedefiningFrontiers in microbiology · 2025
    Review
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  13. Review of the current and potential use of biological and molecular methods for the estimation of the postmortem interval in animals and humans.Journal of veterinary diagnostic investigation : official publication of the American Association of Veterinary Laboratory Diagnosticians, Inc · 2023
    Review
  14. Article
  15. Dental DNA as an Indicator of Post-Mortem Interval (PMI): A Pilot Research.International journal of molecular sciences · 2022
    Article
  16. Article
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4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

9 authors at 2 institutions in 1 country.

Duo PengDepartment of Forensic Genetics, West China School of Basic Science and Forensic Medicine, Sichuan University, Chengdu, 610041, Sichuan, China.
Meili LvDepartment of Forensic Genetics, West China School of Basic Science and Forensic Medicine, Sichuan University, Chengdu, 610041, Sichuan, China.
Zhilong LiDepartment of Forensic Genetics, West China School of Basic Science and Forensic Medicine, Sichuan University, Chengdu, 610041, Sichuan, China.
Huan TianDepartment of Forensic Genetics, West China School of Basic Science and Forensic Medicine, Sichuan University, Chengdu, 610041, Sichuan, China.
ShengQiu QuDepartment of Forensic Genetics, West China School of Basic Science and Forensic Medicine, Sichuan University, Chengdu, 610041, Sichuan, China.
Bo JinDepartment of Forensic Medicine, North Sichuan Medical College, Nanchong, 637000, Sichuan, China.
Bing LongSichuan Police College, Luzhou, 646000, Sichuan, China.
Weibo LiangDepartment of Forensic Genetics, West China School of Basic Science and Forensic Medicine, Sichuan University, Chengdu, 610041, Sichuan, China. liangweibo@scu.edu.cn.ORCID http://orcid.org/0000-0002-8487-3575
Lin ZhangDepartment of Forensic Genetics, West China School of Basic Science and Forensic Medicine, Sichuan University, Chengdu, 610041, Sichuan, China. zhanglin@scu.edu.cn.
Sichuan University · CNNorth Sichuan Medical University · CN

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

Postmortem interval (PMI) determination is an important part of criminal investigations, but it is still subject to uncertainty. Degradation of mRNA in PMI determination has been studied in decays; however, some studies have reported no correlation between PMI and RNA degradation. Thus, we aimed to determine whether RNA quantity was correlated with PMI. Heart and brain tissues were separated from a mouse model of a 0-48 h PMI with 29 time points. We then coextracted the DNA and RNA in one tube with Bioteke coextraction kits and selected some mRNA markers associated with cell oxygen deprivation and apoptosis as target genes, such as hypoxia-associated factor (HAF), apoptosis-inducing factor (AIF), hypoxia-inducible factor 2 alpha (HIF2a), and factor inhibiting HIF (FIH). We measured the quantity of these markers using real-time quantitative PCR (qPCR), and Caspase-3 DNA and 18S were each used for normalization. The results showed that in the heart tissue, the degradation of HIF2a, AIF, and FIH was correlated with PMI, as was the degradation of HIF2a, FIH, and AIF in brain tissue when normalized with Caspase-3 DNA. However, when normalized with 18S, only the degradation of HIF2a in brain tissue was correlated with PMI. Interestingly, the quantity of HAF in brain tissue was found to increase after death with either 18S or Caspase-3 DNA normalization, and it was significantly correlated with 0-48 h PMI. These results indicated that mRNA quantity can be used to determine PMI and that Caspase-3 DNA is feasible for PMI estimation. In summary, we established mathematical models for PMI determination using multiple mRNA markers and multiple tissues and further studies are needed to validate and investigate these markers and mathematical models in human tissues.Duo Peng and Meili Lv contributed equally to this work.

Indexed as

Postmortem ChangesRNA StabilityAnimalsApoptosis Inducing FactorBasic Helix-Loop-Helix ProteinsBiomarkersBrainCaspase 3DNA PrimersEndothelial PAS Domain-Containing Protein 1MiceMice, Inbred C57BLMixed Function OxygenasesModels, AnimalModels, TheoreticalMyocardiumAIFM1 protein, mouseApoptosis Inducing FactorBasic Helix-Loop-Helix ProteinsBiomarkersCasp3 protein, mouseCaspase 3DNA PrimersEndothelial PAS Domain-Containing Protein 1factor inhibiting hypoxia-inducible factor 1, mouseMixed Function OxygenasesNucleic AcidsRibonucleoproteins, Small NuclearRNA, MessengerRNA, Ribosomal, 18SSart1 protein, mouseDegradationDNA and RNA coextractionmRNA markersPMI estimation

Identifiers

PMID31773316
OpenAlexW2990164375

What OpenQuestion holds

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Registered trials

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.