ArticleScientific reports2019
Deconvolution of light sheet microscopy recordings.
Article in Scientific reports, 2019. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 22 papers.
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Who cites it
22 citing papers in PubMed.
- activeDISCO enables large-scale exploration of human brain anatomy by light sheet microscopy.Communications biology · 2026Article
- High-fidelity single-frame computational super-resolution using signal-preserving denoising-enabled deconvolution.Nature communications · 2026Article
- Trans-scale live-imaging of an E5.5 mouse embryo using incubator-type biaxial light-sheet microscopy.Life science alliance · 2025Article
- Article
- A practical guide to light-sheet microscopy for nanoscale imaging: Looking beyond the cell.Journal of applied physics · 2024Article
- Image post-processing for SILMAS: structured illumination light sheet microscopy with axial sweeping.Biomedical optics express · 2024Article
- Axial de-scanning using remote focusing in the detection arm of light-sheet microscopy.Nature communications · 2024Article
- Distortion Correction and Denoising of Light Sheet Fluorescence Images.Sensors (Basel, Switzerland) · 2024Article
- Temporal bone marrow of the rat and its connections to the inner ear.Frontiers in neurology · 2024Article
- Motionless volumetric structured light sheet microscopy.Biomedical optics express · 2023Article
- Smart imaging to empower brain-wide neuroscience at single-cell levels.Brain informatics · 2022Review
- Selective optogenetic control of GNature communications · 2022Article
- Imaging plant cells and organs with light-sheet and super-resolution microscopy.Plant physiology · 2022Article
- Development of Planar Illumination Strategies for Solving Mysteries in the Sub-Cellular Realm.International journal of molecular sciences · 2022Review
- Image Reconstruction in Light-Sheet Microscopy: Spatially Varying Deconvolution and Mixed Noise.Journal of mathematical imaging and vision · 2022Article
- Projective Diffeomorphic Mapping of Molecular Digital Pathology with Tissue MRI.Communications engineering · 2022Article
- Two plus one is almost three: a fast approximation for multi-view deconvolution.Biomedical optics express · 2022Article
- Plant-environment microscopy tracks interactions ofProceedings of the National Academy of Sciences of the United States of America · 2021Article
- Tutorial: methods for three-dimensional visualization of archival tissue material.Nature protocols · 2021Review
- Fluorescence based rapid optical volume screening system (OVSS) for interrogating multicellular organisms.Scientific reports · 2021Article
Corrections and comments
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Authors and funding
8 authors.
Funding
Abstract
We developed a deconvolution software for light sheet microscopy that uses a theoretical point spread function, which we derived from a model of image formation in a light sheet microscope. We show that this approach provides excellent blur reduction and enhancement of fine image details for image stacks recorded with low magnification objectives of relatively high NA and high field numbers as e.g. 2x NA 0.14 FN 22, or 4x NA 0.28 FN 22. For these objectives, which are widely used in light sheet microscopy, sufficiently resolved point spread functions that are suitable for deconvolution are difficult to measure and the results obtained by common deconvolution software developed for confocal microscopy are usually poor. We demonstrate that the deconvolutions computed using our point spread function model are equivalent to those obtained using a measured point spread function for a 10x objective with NA 0.3 and for a 20x objective with NA 0.45.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.