Evidence map›Paper›PMID 31772319›Full record

ArticleScientific reports2019

First molecular detection of Toxoplasma gondii in vegetable samples in China using qualitative, quantitative real-time PCR and multilocus genotyping.

Anna Lass, Liqing Ma, Ioannis Kontogeorgos, Xueyong Zhang, Xiuping Li, Panagiotis Karanis

Abstract read
In one paragraph

Article in Scientific reports, 2019. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 15 papers.

0numbers the graph read from it
0cells of the map it votes in
15citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

15 citing papers in PubMed.

  1. From conventional to biosensor-based detection ofFood and waterborne parasitology · 2026
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  2. Evolution ofJournal of parasitology research · 2026
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  3. Serologic and molecular survey ofOpen veterinary journal · 2025
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  4. Article
  5. Prevalence ofMicroorganisms · 2024
    Article
  6. Article
  7. Foods (Basel, Switzerland) · 2022
    Review
  8. Microorganisms · 2022
    Article
  9. Molecular Characterization ofPathogens (Basel, Switzerland) · 2022
    Article
  10. Article
  11. Review
  12. Article
  13. Review
  14. Review
  15. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

6 authors.

Anna LassDepartment of Tropical Parasitology, Institute of Maritime and Tropical Medicine in Gdynia, Medical University of Gdansk, 9b Powstania Styczniowego Str., 81-519, Gdynia, Poland. anna.lass@gumed.edu.pl.
Liqing MaState Key Laboratory of Plateau Ecology and Agriculture, Center for Biomedicine and Infectious Disease, Qinghai University, 1#Wei'er Road, Qinghai Biological Scientific Estate Garden, Xining, 810016, P.R. China.
Ioannis KontogeorgosState Key Laboratory of Plateau Ecology and Agriculture, Center for Biomedicine and Infectious Disease, Qinghai University, 1#Wei'er Road, Qinghai Biological Scientific Estate Garden, Xining, 810016, P.R. China.
Xueyong ZhangState Key Laboratory of Plateau Ecology and Agriculture, Center for Biomedicine and Infectious Disease, Qinghai University, 1#Wei'er Road, Qinghai Biological Scientific Estate Garden, Xining, 810016, P.R. China.
Xiuping LiState Key Laboratory of Plateau Ecology and Agriculture, Center for Biomedicine and Infectious Disease, Qinghai University, 1#Wei'er Road, Qinghai Biological Scientific Estate Garden, Xining, 810016, P.R. China.
Panagiotis KaranisState Key Laboratory of Plateau Ecology and Agriculture, Center for Biomedicine and Infectious Disease, Qinghai University, 1#Wei'er Road, Qinghai Biological Scientific Estate Garden, Xining, 810016, P.R. China.

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

Toxoplasma gondii infection is becoming increasing problem in China but there is no data concerning contamination of vegetables intended for consumption with this parasite. The aim of the present study was to investigate fresh vegetables originated from open markets located in the Xining City, the Qinghai-Tibet Plateau (QTP), P.R. China for their contamination with T. gondii. A total of 279 fresh vegetable samples were collected and analysed using real-time PCR assay targeting B1 gene and multilocus genotyping. T. gondii DNA was found in 10 (3.6%) samples tested; eight of them represented T. gondii type I and remaining two T. gondii type II. The approximate level of contamination of positive vegetables samples, estimated based on quantitative real-time PCR (qPCR), ranged between less than one and 27000 T. gondii oocysts per sample, with majority not exceeding several oocysts per sample. The results of the study confirmed that T. gondii is present in vegetables offered in open markets in the Qinghai province, P.R. China; eating them unwashed and raw may therefore pose a threat to consumers. This is the first investigation describing T. gondii detection in fresh vegetables intended for consumption collected from the territory of P.R. China using sensitive molecular tools.

Indexed as

Food ParasitologyChinaDNA, ProtozoanMultilocus Sequence TypingReal-Time Polymerase Chain ReactionToxoplasmaVegetablesDNA, Protozoan

Identifiers

PMID31772319
PMCPMC6879479

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Registered trials

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.