ArticleScientific reports2019
Enzyme-Assisted Photoinjection of Megadalton Molecules into Intact Plant Cells Using Femtosecond Laser Amplifier.
Article in Scientific reports, 2019. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 6 papers.
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Who cites it
6 citing papers in PubMed, 18 citations in OpenAlex.
- Acidic pH Condition Enhances Femtosecond Laser Photoinjection of Plasmid DNA into Intact Tobacco BY‑2.ACS omega · 2025Article
- Article
- Cell viability assessment associated with a contact of gas bubbles produced by femtosecond laser breakdown in cell culture media.Scientific reports · 2022Article
- Identification of Single Yeast Budding Using Impedance Cytometry with a Narrow Electrode Span.Sensors (Basel, Switzerland) · 2022Article
- Laser-based molecular delivery and its applications in plant science.Plant methods · 2022Review
- Hechtian Strands Transmit Cell Wall Integrity Signals in Plant Cells.Plants (Basel, Switzerland) · 2020Article
Corrections and comments
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Authors and funding
7 authors at 3 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Femtosecond laser photoporation has become a popular method to deliver various kinds of molecules such as genes, proteins, and fluorescent dyes into single mammalian cells. However, this method is not easily applied to plant cells because their cell wall and turgor pressure prevent the delivery, especially for larger molecules than the mesh size of the cell wall. This work is the first demonstration of the efficient photoinjection of megadalton molecules into a cytoplasm of an intact single plant cell by employing a femtosecond laser amplifier under moderate enzyme treatment conditions. The intense femtosecond laser pulse effectively formed a pore on the cell wall and membrane of Tobacco BY-2, and 2 MDa dextran molecules were introduced through the pore. Along with the pore formation, induced mechanical tensile stresses on BY-2 cells were considered to increase permeability of the cell membrane and enhance the uptake of large molecules. Moreover, the moderate enzyme treatment partially degraded the cell wall thereby facilitating the increase of the molecular introduction efficiency.
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Registered trials
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