Evidence map›Paper›PMID 31771979›Full record

ArticleThe Journal of biological chemistry2020

Moonlighting matrix metalloproteinase substrates: Enhancement of proinflammatory functions of extracellular tyrosyl-tRNA synthetase upon cleavage.

Parker G Jobin, Nestor Solis, Yoan Machado, Peter A Bell, Simran K Rai, Nam Hoon Kwon, Sunghoon Kim, Christopher M Overall, Georgina S Butler

Open access · hybridAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in The Journal of biological chemistry, 2020. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 13 papers.

0numbers the graph read from it
0cells of the map it votes in
13citing papers in PubMed
1.1field-weighted citation impact, top 22% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

13 citing papers in PubMed, 21 citations in OpenAlex.

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4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

9 authors at 2 institutions in 3 countries.

Parker G JobinDepartment of Biochemistry and Molecular Biology, University of British Columbia, Vancouver, British Columbia V6T 1Z3, Canada; Centre for Blood Research, University of British Columbia, Vancouver, British Columbia V6T 1Z3, Canada.
Nestor SolisCentre for Blood Research, University of British Columbia, Vancouver, British Columbia V6T 1Z3, Canada; Department of Oral Biological and Medical Sciences, Faculty of Dentistry, University of British Columbia, Vancouver, British Columbia V6T 1Z3, Canada.
Yoan MachadoCentre for Blood Research, University of British Columbia, Vancouver, British Columbia V6T 1Z3, Canada; Department of Oral Biological and Medical Sciences, Faculty of Dentistry, University of British Columbia, Vancouver, British Columbia V6T 1Z3, Canada.
Peter A BellCentre for Blood Research, University of British Columbia, Vancouver, British Columbia V6T 1Z3, Canada; Department of Oral Biological and Medical Sciences, Faculty of Dentistry, University of British Columbia, Vancouver, British Columbia V6T 1Z3, Canada.
Simran K RaiCentre for Blood Research, University of British Columbia, Vancouver, British Columbia V6T 1Z3, Canada; Graduate Program in Bioinformatics, University of British Columbia, Vancouver, British Columbia V5T 4S6, Canada.
Nam Hoon KwonCollege of Pharmacy, Seoul National University, 151-742, Seoul, Republic of Korea; Medicinal Bioconvergence Research Center, Seoul National University, 151-742, Seoul, Republic of Korea.
Sunghoon KimCollege of Pharmacy, Seoul National University, 151-742, Seoul, Republic of Korea; Medicinal Bioconvergence Research Center, Seoul National University, 151-742, Seoul, Republic of Korea.
Christopher M OverallDepartment of Biochemistry and Molecular Biology, University of British Columbia, Vancouver, British Columbia V6T 1Z3, Canada; Centre for Blood Research, University of British Columbia, Vancouver, British Columbia V6T 1Z3, Canada; Department of Oral Biological and Medical Sciences, Faculty of Dentistry, University of British Columbia, Vancouver, British Columbia V6T 1Z3, Canada. Electronic address: chris.overall@ubc.ca.
Georgina S ButlerCentre for Blood Research, University of British Columbia, Vancouver, British Columbia V6T 1Z3, Canada; Department of Oral Biological and Medical Sciences, Faculty of Dentistry, University of British Columbia, Vancouver, British Columbia V6T 1Z3, Canada.
University of British Columbia · CASeoul National University · KR

Funding

CIHR FDN-148408CIHR MOP-111055
6 · The paper itself

Abstract

Tyrosyl-tRNA synthetase ligates tyrosine to its cognate tRNA in the cytoplasm, but it can also be secreted through a noncanonical pathway. We found that extracellular tyrosyl-tRNA synthetase (YRS) exhibited proinflammatory activities. In addition to acting as a monocyte/macrophage chemoattractant, YRS initiated signaling through Toll-like receptor 2 (TLR2) resulting in NF-κB activation and release of tumor necrosis factor α (TNFα) and multiple chemokines, including MIP-1α/β, CXCL8 (IL8), and CXCL1 (KC) from THP1 monocyte and peripheral blood mononuclear cell-derived macrophages. Furthermore, YRS up-regulated matrix metalloproteinase (MMP) activity in a TNFα-dependent manner in M0 macrophages. Because MMPs process a variety of intracellular proteins that also exhibit extracellular moonlighting functions, we profiled 10 MMPs for YRS cleavage and identified 55 cleavage sites by

Indexed as

ChemokinesChemotaxisEnzyme StabilityExtracellular SpaceHumansInflammation MediatorsMacrophagesMatrix MetalloproteinasesModels, BiologicalMonocytesNF-kappa BSignal TransductionSubstrate SpecificityTHP-1 CellsToll-Like Receptor 2Tumor Necrosis Factor-alphaChemokinesInflammation MediatorsMatrix MetalloproteinasesNF-kappa BToll-Like Receptor 2Tumor Necrosis Factor-alphaTyrosineTyrosine-tRNA Ligaseaminoacyl tRNA synthetaseinflammationinnate immunitymacrophagematrix metalloproteinase (MMP)moonlighting proteinsmultifunctional proteinproteolysistoll-like receptor (TLR)

Identifiers

PMID31771979
PMCPMC7039567
OpenAlexW2989661513

What OpenQuestion holds

Textmetadata
LicenceCC BY
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.