ArticlePLoS neglected tropical diseases2019
Comparative analysis of small RNAs released by the filarial nematode Litomosoides sigmodontis in vitro and in vivo.
Article in PLoS neglected tropical diseases, 2019. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 18 papers.
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Who cites it
18 citing papers in PubMed, 27 citations in OpenAlex.
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- PANDORA-Seq unveils the hidden small noncoding RNA landscape in atherosclerosis of LDL receptor-deficient mice.Journal of lipid research · 2023Article
- How to train your myeloid cells: a way forward for helminth vaccines?Frontiers in immunology · 2023Review
- MicroRNAs: Harbingers and shapers of periodontal inflammation.Seminars in cell & developmental biology · 2022Review
- Nematode microRNAs can Individually Regulate Interferon Regulatory Factor 4 and mTOR in Differentiating T Helper 2 Lymphocytes and Modulate Cytokine Production in Macrophages.Frontiers in molecular biosciences · 2022Article
- Human filariasis-contributions of the Litomosoides sigmodontis and Acanthocheilonema viteae animal model.Parasitology research · 2021Review
- Helminth extracellular vesicles: Interactions with the host immune system.Molecular immunology · 2021Review
- Secretory microRNA Profiles of Third- and Fourth-StagePathogens (Basel, Switzerland) · 2021Article
- RNAs on the Go: Extracellular Transfer in Insects with Promising Prospects for Pest Management.Plants (Basel, Switzerland) · 2021Review
- Expression profiling of Echinococcus multilocularis miRNAs throughout metacestode development in vitro.PLoS neglected tropical diseases · 2021Article
- Role ofFrontiers in cellular and infection microbiology · 2021Article
- Extracellular vesicles from Heligmosomoides bakeri and Trichuris muris contain distinct microRNA families and small RNAs that could underpin different functions in the host.International journal for parasitology · 2020Article
- Trichinella spiralis secretes abundant unencapsulated small RNAs with potential effects on host gene expression.International journal for parasitology · 2020Article
- ADAM (a Disintegrin and Metalloproteinase) 15 Deficiency Exacerbates Ang II (Angiotensin II)-Induced Aortic Remodeling Leading to Abdominal Aortic Aneurysm.Arteriosclerosis, thrombosis, and vascular biology · 2020Article
- Disentangling sRNA-Seq data to study RNA communication between species.Nucleic acids research · 2020Article
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Authors and funding
11 authors at 3 institutions in 2 countries.
Funding
Abstract
backgroundThe release of small non-coding RNAs (sRNAs) has been reported in parasitic nematodes, trematodes and cestodes of medical and veterinary importance. However, little is known regarding the diversity and composition of sRNAs released by different lifecycle stages and the portion of sRNAs that persist in host tissues during filarial infection. This information is relevant to understanding potential roles of sRNAs in parasite-to-host communication, as well as to inform on the location within the host and time point at which they can be detected. METHODOLOGY AND PRINCIPAL
findingsWe have used small RNA (sRNA) sequencing analysis to identify sRNAs in replicate samples of the excretory-secretory (ES) products of developmental stages of the filarial nematode Litomosoides sigmodontis in vitro and compare this to the parasite-derived sRNA detected in host tissues. We show that all L. sigmodontis developmental stages release RNAs in vitro, including ribosomal RNA fragments, 5'-derived tRNA fragments (5'-tRFs) and, to a lesser extent, microRNAs (miRNAs). The gravid adult females (gAF) produce the largest diversity and abundance of miRNAs in the ES compared to the adult males or microfilariae. Analysis of sRNAs detected in serum and macrophages from infected animals reveals that parasite miRNAs are preferentially detected in vivo, compared to their low levels in the ES products, and identifies miR-92-3p and miR-71-5p as L. sigmodontis miRNAs that are stably detected in host cells in vivo.
conclusionsOur results suggest that gravid adult female worms secrete the largest diversity of extracellular sRNAs compared to adult males or microfilariae. We further show differences in the parasite sRNA biotype distribution detected in vitro versus in vivo. We identify macrophages as one reservoir for parasite sRNA during infection, and confirm the presence of parasite miRNAs and tRNAs in host serum during patent infection.
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