ArticlePlant biotechnology journal2020
Multiplex CRISPR/Cas9-mediated metabolic engineering increases soya bean isoflavone content and resistance to soya bean mosaic virus.
Article in Plant biotechnology journal, 2020. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 119 papers, 1 of them a synthesis that pooled it.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
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Who cites it
119 citing papers in PubMed, 1 synthesis or guideline pooled it, 317 citations in OpenAlex.
- Development and research trends of stay-green biology in legumes: a bibliometric and visual analysis over three decades.Frontiers in genetics · 2026Pooled it
- Advances in CRISPR multiplex gene editing to map and modify stress-responsive transcription factor networks for crop improvement.Plant cell reports · 2026Review
- Unveiling Dynamic Defense Pathways: Transcriptome Analysis of Phenylpropanoids and (Iso)flavonoids in Cowpea Leaves Post-CABMV Inoculation.Biochemical genetics · 2026Article
- Industrial Bioreactor Technologies for Modern Biomanufacturing: Recent Applications in Microbial, Mammalian, and Plant-Based Systems.Molecular biotechnology · 2026Review
- Identification and Expression Profile of the Chalcone Synthase (International journal of molecular sciences · 2026Article
- Next-Generation Metabolic Engineering of Capsaicinoids Biosynthesis in Chilli Pepper: Bridging Genomic Insights to Biotechnological Applications.Biotech (Basel (Switzerland)) · 2026Review
- GmMYB4 Positively Regulates Isoflavone Biosynthesis via the GmMAPK6-GmMYB4-MBW Module in Soybean.Plant biotechnology journal · 2026Article
- Multiplex gene editing drives revolution in crop breeding: overlaid editing of multiple genes and customization of complex traits.Advanced biotechnology · 2026Review
- Enhancing heritable genome editing in soybean by optimizing promoter combinations for the LbCas12a system.BMC plant biology · 2026Article
- Flavonoids in Medicine and Food Homology Substances: Structure-Activity Relationship, Application Challenges, and Cutting-Edge Technological Breakthroughs.Foods (Basel, Switzerland) · 2026Review
- Challenges and Opportunities with CRISPR-Based Genome Editing in Legume Crops.Functional & integrative genomics · 2026Review
- Flavonoids in Plant Salt Stress Responses: Biosynthesis, Regulation, Functions, and Signaling Networks.Plants (Basel, Switzerland) · 2026Review
- CRISPR/Cas9 in soybean research: a measured leap toward genetic refinement.Frontiers in genome editing · 2026Review
- A review of flavonoids at the crossroads of plant defense: integrating biotic and abiotic stress tolerance through AI- and CRISPR/Cas-guided metabolic reprogramming.Frontiers in plant science · 2026Review
- A natural variant of anProceedings of the National Academy of Sciences of the United States of America · 2025Article
- Advancements in Molecular Breeding Techniques for Soybeans.Plants (Basel, Switzerland) · 2025Review
- A balance between glycitein and glyceollins governed by isoflavone 6-hydroxylase confers soybean resistance toProceedings of the National Academy of Sciences of the United States of America · 2025Article
- MutMap-Based Cloning of a Soybean Mosaic Virus Resistance Gene.Plants (Basel, Switzerland) · 2025Article
- Transcriptome analysis reveals key pathways and regulatory networks involved in soybean's early resistance to Peronospora manshurica.BMC plant biology · 2025Article
- Revolution and advances in gene editing and genomics technology for developing climate-resilient legume crops: developments and prospects.Plant molecular biology · 2025Review
59 more citing papers are in PubMed but not listed here.
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
9 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Isoflavonoids, which include a variety of secondary metabolites, are derived from the phenylpropanoid pathway and are distributed predominantly in leguminous plants. These compounds play a critical role in plant-environment interactions and are beneficial to human health. Isoflavone synthase (IFS) is a key enzyme in isoflavonoid synthesis and shares a common substrate with flavanone-3-hydroxylase (F3H) and flavone synthase II (FNS II). In this study, CRISPR/Cas9-mediated multiplex gene-editing technology was employed to simultaneously target GmF3H1, GmF3H2 and GmFNSII-1 in soya bean hairy roots and plants. Various mutation types and frequencies were observed in hairy roots. Higher mutation efficiencies were found in the T
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.