Evidence map›Paper›PMID 31768992›Full record

ArticleMethods in molecular biology (Clifton, N.J.)2020

Purification and Reconstitution of the S. cerevisiae TRAMP and Ski Complexes for Biochemical and Structural Studies.

Achim Keidel, Elena Conti, Sebastian Falk

Erratum issuedAbstract read
PubMed Publisher
In one paragraph

Article in Methods in molecular biology (Clifton, N.J.), 2020. The graph could read no effect estimate from its abstract, so it casts no vote on the map. An erratum has been issued. Cited by 1 paper.

0numbers the graph read from it
0cells of the map it votes in
1citing papers in PubMed
–field-weighted citation impact, top 74% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

1 citing paper in PubMed, 1 citations in OpenAlex.

  1. Article
4 · The record

Corrections and comments

5 · Who and what money

Authors and funding

3 authors at 1 institution in 2 countries.

Achim KeidelDepartment of Structural Cell Biology, Max-Planck-Institute of Biochemistry, Martinsried, Germany.
Elena ContiDepartment of Structural Cell Biology, Max-Planck-Institute of Biochemistry, Martinsried, Germany. conti@biochem.mpg.de.
Sebastian FalkDepartment of Structural Cell Biology, Max-Planck-Institute of Biochemistry, Martinsried, Germany. sebastian.falk@univie.ac.at.
Max Planck Institute of Biochemistry · DE

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

The RNA exosome is a macromolecular machine that degrades a large variety of RNAs from their 3'-end. It comprises the major 3'-to-5' exonuclease in the cell, completely degrades erroneous and overly abundant RNAs, and is also involved in the precise processing of RNAs. To degrade transcripts both specifically and efficiently the exosome functions together with compartment-specific cofactors. In the yeast S. cerevisiae, the exosome associates with the Ski complex in the cytoplasm and with Mtr4 alone or with Mtr4 as part of the TRAMP complex in the nucleus. Here we describe how to produce, purify, and assemble the Ski and TRAMP complexes from S. cerevisiae.

Indexed as

AnimalsCell NucleusCytoplasmExosomesRNARNA, FungalSaccharomyces cerevisiaeSaccharomyces cerevisiae ProteinsSerine EndopeptidasesSf9 CellsRNARNA, FungalSaccharomyces cerevisiae ProteinsSerine Endopeptidasestunicate retinoic acid-inducible modular proteaseAir2ExosomeMtr4Ski2Ski3Ski8Ski complexTRAMPTrf4

Identifiers

PMID31768992
OpenAlexW2990955595

What OpenQuestion holds

Textmetadata
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.