Evidence map›Paper›PMID 31768989›Full record

ArticleMethods in molecular biology (Clifton, N.J.)2020

Reconstitution of S. cerevisiae RNA Exosome Complexes Using Recombinantly Expressed Proteins.

John C Zinder, Christopher D Lima

Open access · greenAbstract read
In one paragraph

Article in Methods in molecular biology (Clifton, N.J.), 2020. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 1 paper.

0numbers the graph read from it
0cells of the map it votes in
1citing papers in PubMed
–field-weighted citation impact, top 77% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

1 citing paper in PubMed, 1 citations in OpenAlex.

  1. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

2 authors at 2 institutions in 1 country.

John C ZinderTri-Institutional Training Program in Chemical Biology, Memorial Sloan Kettering Cancer Center, New York, NY, USA.
Christopher D LimaStructural Biology Program, Sloan Kettering Institute, Memorial Sloan Kettering Cancer Center, New York, NY, USA. limac@mskcc.org.
Howard Hughes Medical Institute · USTri-Institutional PhD Program in Chemical Biology · US

Funding

X-RAY CRYSTALLOGRAPHYP30CA008748 · NCI · SLOAN-KETTERING INSTITUTE FOR CANCER RES · PI Nicholas D Socci · 1985 to 2026
$347.4M
Structural studies of RNA processing and ubiquitin-like protein modificationR35GM118080 · NIGMS · SLOAN-KETTERING INST CAN RESEARCH · PI CHRISTOPHER D. LIMA · 2016 to 2026
$5.0M
Structural studies of ubiquitin-like protein modificationR01GM065872 · NIGMS · WEILL MEDICAL COLLEGE OF CORNELL UNIV · PI LIMA, CHRISTOPHER D. · 2002 to 2015
$4.9M
NCI NIH HHS P30 CA008748NIGMS NIH HHS R01 GM065872NIGMS NIH HHS R35 GM118080
6 · The paper itself

Abstract

3' to 5' RNA degradation is primarily catalyzed by the RNA exosome subunits Dis3 and Rrp6 in the nucleus of Saccharomyces cerevisiae. These enzymes form a complex with the nine-subunit noncatalytic core (Exo9) to carry out their functions in vivo. Protein cofactors Rrp47, Mpp6, and the Mtr4 RNA helicase also assist the complex by modulating its activities and/or recruiting it to specific RNAs for processing or degradation. Here we present our preferred strategy for reconstituting RNA exosomes from S. cerevisiae using purified, recombinantly expressed components.

Indexed as

DEAD-box RNA HelicasesDNA-Binding ProteinsExoribonucleasesExosome Multienzyme Ribonuclease ComplexExosomesNuclear ProteinsRNA-Binding ProteinsRNA, FungalRNA StabilitySaccharomyces cerevisiaeSaccharomyces cerevisiae ProteinsDEAD-box RNA HelicasesDNA-Binding ProteinsExoribonucleasesExosome Multienzyme Ribonuclease ComplexLRP1 protein, S cerevisiaeMpp6 protein, S cerevisiaeMTR4 protein, S cerevisiaeNuclear ProteinsRNA-Binding ProteinsRNA, FungalSaccharomyces cerevisiae ProteinsBudding yeastExoribonucleaseRNA decayRNA exosome

Identifiers

PMID31768989
PMCPMC8596991
OpenAlexW2991106200

What OpenQuestion holds

Textmetadata
LicenceTDM
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.