Evidence map›Paper›PMID 31768983›Full record

ArticleMethods in molecular biology (Clifton, N.J.)2020

Affinity Proteomic Analysis of the Human Exosome and Its Cofactor Complexes.

Kinga Winczura, Michal Domanski, John LaCava

Erratum issuedAbstract read
PubMed Publisher
In one paragraph

Article in Methods in molecular biology (Clifton, N.J.), 2020. The graph could read no effect estimate from its abstract, so it casts no vote on the map. An erratum has been issued. Cited by 4 papers.

0numbers the graph read from it
0cells of the map it votes in
4citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

4 citing papers in PubMed.

  1. Article
  2. Article
  3. Article
  4. Review
4 · The record

Corrections and comments

5 · Who and what money

Authors and funding

3 authors.

Kinga WinczuraSchool of Biosciences, College of Life and Environmental Sciences, University of Birmingham, Birmingham, UK.
Michal DomanskiDepartment of Chemistry and Biochemistry, University of Bern, Bern, Switzerland.
John LaCavaLaboratory of Cellular and Structural Biology, The Rockefeller University, New York, NY, USA. jlacava@rockefeller.edu.

Funding

Demonstrating interactome-level connections between Alzheimer disease, LINE-1 retrotransposons, and cellular senescenceR01GM126170 · NIGMS · ROCKEFELLER UNIVERSITY · PI LACAVA, JOHN · 2018 to 2021
$2.4M
NIGMS NIH HHS R01 GM126170
6 · The paper itself

Abstract

In humans, the RNA exosome consists of an enzymatically inactive nine-subunit core, with ribonucleolytic activity contributed by additional components. Several cofactor complexes also interact with the exosome-these enable the recruitment of, and specify the activity upon, diverse substrates. Affinity capture coupled with mass spectrometry has proven to be an effective means to identify the compositions of RNA exosomes and their cofactor complexes: here, we describe a general experimental strategy for proteomic characterization of macromolecular complexes, applied to the exosome and an affiliated adapter protein, ZC3H18.

Indexed as

Cell LineExosome Multienzyme Ribonuclease ComplexExosomesHEK293 CellsHumansProteomicsRNARNA-Binding ProteinsExosome Multienzyme Ribonuclease ComplexRNARNA-Binding ProteinsAffinity captureAffinity proteomicsCofactorsExosomeMass spectrometry

Identifiers

PMID31768983

What OpenQuestion holds

Textmetadata
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.