Purification and In Vitro Analysis of the Exosome Cofactors Nrd1-Nab3 and Trf4-Air2.

Odil Porrua

Abstract read

In one paragraph

Article in Methods in molecular biology (Clifton, N.J.), 2020. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

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0citing papers in PubMed

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1 · What the graph read from it

What it found

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2 · The registry

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3 · Its place in the literature

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0 citing papers in PubMed, 0 citations in OpenAlex.

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4 · The record

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5 · Who and what money

Authors and funding

1 author at 1 institution in 1 country.

Odil PorruaInstitut Jacques Monod-UMR7592, CNRS, Université de Paris, Paris, France. odil.porrua@ijm.fr.

Université Paris Cité · FR

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

In many eukaryotic organisms from yeast to human, the exosome plays an important role in the control of pervasive transcription and in non-coding RNA (ncRNA) processing and quality control by trimming precursor RNAs and degrading aberrant transcripts. In Saccharomyces cerevisiae this function is enabled by the interaction of the exosome with several cofactors: the Nrd1-Nab3 heterodimer and the Trf4-Air2-Mtr4 (TRAMP4) complex. Nrd1 and Nab3 are RNA binding proteins that recognize specific motifs enriched in the target ncRNAs, whereas TRAMP4 adds polyA tails at the 3' end of transcripts and stimulates RNA degradation by the exosome. This chapter provides protocols for the purification of recombinant forms of these exosome cofactors and for the in vitro assessment of their activity.

Indexed as

Adaptor Proteins, Signal TransducingDNA-Directed DNA PolymeraseExosomesGene Expression Regulation, FungalNuclear ProteinsPolyadenylationRNA-Binding ProteinsRNA, FungalRNA StabilitySaccharomyces cerevisiaeSaccharomyces cerevisiae ProteinsTranscription, GeneticAdaptor Proteins, Signal TransducingAir2 protein, S cerevisiaeDNA-Directed DNA PolymeraseNAB3 protein, S cerevisiaeNRD1 protein, S cerevisiaeNuclear ProteinsPAP2 protein, S cerevisiaeRNA-Binding ProteinsRNA, FungalSaccharomyces cerevisiae ProteinsExosome cofactorsNon-coding RNAsNrd1-Nab3Pervasive transcriptionRNA-binding proteinsRNA polyadenylationRNA quality controlTrf4-Air2

Identifiers

PMID31768982

DOI10.1016/j.pep.2005.01.016

OpenAlexW2989888013

What OpenQuestion holds

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.