ArticlePlant molecular biology2020
A U-box E3 ubiquitin ligase OsPUB67 is positively involved in drought tolerance in rice.
Article in Plant molecular biology, 2020. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 49 papers.
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Who cites it
49 citing papers in PubMed, 104 citations in OpenAlex.
- Ubiquitin ligase HcPUB30 targets HcWRKY1 to regulate monoterpenoids synthesis in Hedychium coronarium.BMC plant biology · 2026Article
- Genome-Wide Identification and Expression Analysis of the U-Box E3 Ubiquitin Ligase Gene Family Related to Monoterpene Indole Alkaloids Biosynthesis inInternational journal of molecular sciences · 2026Article
- Identification of SNPs and Candidate Genes Associated with Major Drought Tolerance QTL on Wheat Chromosome 4A.Plants (Basel, Switzerland) · 2026Article
- Harnessing Antioxidants for Abiotic Stress Management: Mechanistic Insights and Prospects for Sustainable Agriculture.Antioxidants (Basel, Switzerland) · 2026Review
- Investigating the Function of TaUBX57 in Enhancing Abiotic Stress Tolerance in Wheat.International journal of molecular sciences · 2025Article
- Deciphering the regulatory network of carbon isotope discrimination in bread wheat through genome-wide association studies and genomic prediction.TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik · 2025Article
- Enhancing drought resilience in crops: mechanistic approaches in the face of climate challenge.Plant molecular biology · 2025Review
- Strategies to develop climate-resilient chili peppers: transcription factor optimization through genome editing.Planta · 2025Review
- Genome-Wide Identification, Characterization, and Expression Analysis of the U-Box Gene Family in Cucumber (Plants (Basel, Switzerland) · 2025Article
- Ubiquitin Ligase GenePlants (Basel, Switzerland) · 2025Article
- Genome-wide association analysis in identification of superior haplotypes for vegetative stage drought stress tolerance in rice.Physiology and molecular biology of plants : an international journal of functional plant biology · 2025Article
- NIN-like proteins NLP8 negatively regulate drought resistance in Arabidopsis by regulating the expression level of PUB23.Plant cell reports · 2025Article
- Wheat E3 ligasePhysiology and molecular biology of plants : an international journal of functional plant biology · 2025Article
- Association mapping and candidate gene identification for drought tolerance in sorghum.Frontiers in plant science · 2025Article
- CRISPR/Cas9: a sustainable technology to enhance climate resilience in major Staple Crops.Frontiers in genome editing · 2025Review
- Genome-wide identification and expression analysis of soybean bHLH transcription factor and its molecular mechanism on grain protein synthesis.Frontiers in plant science · 2025Article
- Genome-Wide Identification and Expression Analysis of the Alfalfa (International journal of molecular sciences · 2024Article
- Arabidopsis ubiquitin ligase PUB41 positively regulates ABA-mediated seed dormancy and drought response.Physiology and molecular biology of plants : an international journal of functional plant biology · 2024Article
- Deciphering the mechanism of E3 ubiquitin ligases in plant responses to abiotic and biotic stresses and perspectives on PROTACs for crop resistance.Plant biotechnology journal · 2024Review
- Functionality of Reactive Oxygen Species (ROS) in Plants: Toxicity and Control inPlants (Basel, Switzerland) · 2024Review
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Authors and funding
8 authors at 3 institutions in 1 country.
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Abstract
key messageOsPUB67, a U-box E3 ubiquitin ligase, may interact with two drought tolerance negative regulators (OsRZFP34 and OsDIS1) and improve drought tolerance by enhancing the reactive oxygen scavenging ability and stomatal closure. E3 ubiquitin ligases are major components of the ubiquitination cascade and contribute to the biotic and abiotic stress response in plants. In the present study, we show that a rice drought responsive gene, OsPUB67, encoding the U-box E3 ubiquitin ligase was significantly induced by drought, salt, cold, JA, and ABA, and was expressed in nuclei, cytoplasm, and membrane systems. This distribution of expression suggests a significant role for OsPUB67 in a wide range of biological processes and abiotic stress response. Over-expression of OsPUB67 improved drought stress tolerance by enhancing the reactive oxygen scavenging ability and stomatal closure. Bimolecular fluorescence complementation assays revealed that a few E2s interacted with OsPUB67 with unique functional implications in different cell components. Further evidence showed that several E3 ubiquitin ligases interacted with OsPUB67, especially OsRZFP34 and OsDIS1, which are negative regulators of drought tolerance. This interaction on the stomata implied OsPUB67 might function as a heterodimeric ubiquitination complex in response to drought stress. Comprehensive transcriptome analysis revealed OsPUB67 participated in regulating genes involved in the abiotic stress response and transcriptional regulation in an ABA-dependent manner. Our findings revealed OsPUB67 mediated a multilayered complex drought stress tolerance mechanism.
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