Evidence map›Paper›PMID 31768650›Full record

ArticleMikrochimica acta2019

Multiplexed aptasensing of food contaminants by using terminal deoxynucleotidyl transferase-produced primer-triggered rolling circle amplification: application to the colorimetric determination of enrofloxacin, lead (II), Escherichia coli O157:H7 and tropomyosin.

Yumei Du, Yangyang Zhou, Yanli Wen, Xiaojun Bian, Yuanyuan Xie, Weijia Zhang, Gang Liu, Juan Yan

Abstract read
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In one paragraph

Article in Mikrochimica acta, 2019. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 6 papers.

0numbers the graph read from it
0cells of the map it votes in
6citing papers in PubMed
1.4field-weighted citation impact, top 18% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

6 citing papers in PubMed, 33 citations in OpenAlex.

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4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

8 authors at 3 institutions in 2 countries.

Yumei DuLaboratory of Quality and Safety Risk Assessment for Aquatic Products on Storage and Preservation (Shanghai), Ministry of Agriculture; Shanghai Engineering Research Center of Aquatic-Product Process & Preservation; College of Food Science and Technology, Shanghai Ocean University, Shanghai, 201306, China.
Yangyang ZhouLaboratory of Quality and Safety Risk Assessment for Aquatic Products on Storage and Preservation (Shanghai), Ministry of Agriculture; Shanghai Engineering Research Center of Aquatic-Product Process & Preservation; College of Food Science and Technology, Shanghai Ocean University, Shanghai, 201306, China.
Yanli WenLaboratory of Biometrology, Division of Chemistry and Ionizing Radiation Measurement Technology, Shanghai Institute of Measurement and Testing Technology, Shanghai, 201203, China.
Xiaojun BianLaboratory of Quality and Safety Risk Assessment for Aquatic Products on Storage and Preservation (Shanghai), Ministry of Agriculture; Shanghai Engineering Research Center of Aquatic-Product Process & Preservation; College of Food Science and Technology, Shanghai Ocean University, Shanghai, 201306, China.
Yuanyuan XieLaboratory of Quality and Safety Risk Assessment for Aquatic Products on Storage and Preservation (Shanghai), Ministry of Agriculture; Shanghai Engineering Research Center of Aquatic-Product Process & Preservation; College of Food Science and Technology, Shanghai Ocean University, Shanghai, 201306, China.
Weijia ZhangFifth People's Hospital of Shanghai and Institutes of Biomedical Sciences, and State Key Laboratory of Molecular Engineering, Fudan University, Shanghai, 200032, China.
Gang LiuLaboratory of Biometrology, Division of Chemistry and Ionizing Radiation Measurement Technology, Shanghai Institute of Measurement and Testing Technology, Shanghai, 201203, China. liug@simt.com.cn.ORCID 0000-0002-0859-3985
Juan YanLaboratory of Quality and Safety Risk Assessment for Aquatic Products on Storage and Preservation (Shanghai), Ministry of Agriculture; Shanghai Engineering Research Center of Aquatic-Product Process & Preservation; College of Food Science and Technology, Shanghai Ocean University, Shanghai, 201306, China. j-yan@shou.edu.cn.ORCID 0000-0003-4529-2046
Ministère de l'Agriculture et de la Souveraineté alimentaire · FRShanghai Institute of Measurement and Testing Technology · CNFudan University · CN

Funding

National Natural Science Foundation of China 21775102National Natural Science Foundation of China 21775104Shanghai Rising-Star Program 16QB1403100
6 · The paper itself

Abstract

A colorimetric assay is described for simultaneous detection of multiple analytes related to food safety. It is based on the use of a sandwich aptasensor and terminal deoxynucleotidyl transferase (TdT) which produces a primer for subsequent rolling circle amplification (RCA). Two split aptamer fragments (Apt1 and Apt2) are firstly immobilized, Apt1 on gold nanoparticles (AuNPs), and Apt2 on magnetic beads (MBs). They are then used in a sandwich aptasensor. In the presence of analyte, two probes could specifically recognize target and form a ternary assembly, and the magnetic beads also act to separate rapidly and enrich the target. Then, the extension of template-free DNA is triggered by TdT at the exposed 3'-hydroxy terminals of Apt1. This produces polyA sequences that serve as primers for subsequent RCA. The product of RCA is hybridized with a complementary horse radish peroxidase (HRP) DNA probe. HRP catalyzes the H

Indexed as

AnimalsAptamers, NucleotideArmoraciaBenzidinesBiosensing TechniquesColorimetryColoring AgentsDNADNA NucleotidylexotransferaseEnrofloxacinEscherichia coli O157Food ContaminationGoldHorseradish PeroxidaseHydrogen PeroxideLead3,3',5,5'-tetramethylbenzidineAptamers, NucleotideBenzidinesColoring AgentsDNADNA NucleotidylexotransferaseEnrofloxacinGoldHorseradish PeroxidaseHydrogen PeroxideLeadTropomyosinAntibioticsAptasensorBiodetectionFood safety detectionGold nanoparticlesIsothermal nucleic acid amplificationMagnetic beadsSignal amplification method

Identifiers

PMID31768650
OpenAlexW2990333927

What OpenQuestion holds

Textmetadata
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.