ArticleMolecular metabolism2019
Establishment of a high-resolution 3D modeling system for studying pancreatic epithelial cell biology in vitro.
Article in Molecular metabolism, 2019. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 21 papers.
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Who cites it
21 citing papers in PubMed, 26 citations in OpenAlex.
- Charting Endocrine Progenitors Across Species and Organs.Advanced science (Weinheim, Baden-Wurttemberg, Germany) · 2026Article
- Stem cell-based therapies for type 1 diabetes: Progress in differentiation, clinical translation, and immune protection.Animal models and experimental medicine · 2026Review
- Benchmarking porcine pancreatic ductal organoids for drug screening applications.EMBO molecular medicine · 2025Article
- Generation of human iPSC-derived pancreatic organoids to study pancreas development and disease.F1000Research · 2025Article
- Towards a better understanding of diabetes mellitus using organoid models.Nature reviews. Endocrinology · 2023Review
- Heterogeneity of Islet Cells during Embryogenesis and Differentiation.Diabetes & metabolism journal · 2023Review
- NEUROD2 function is dispensable for human pancreatic β cell specification.Frontiers in endocrinology · 2023Article
- Synaptotagmin-13 orchestrates pancreatic endocrine cell egression and islet morphogenesis.Nature communications · 2022Article
- Development of scaffold-free vascularized pancreatic beta-islets in vitro models by the anchoring of cell lines to a bioligand-functionalized gelatine substrate.Journal of materials science. Materials in medicine · 2022Article
- Islet organoid as a promising model for diabetes.Protein & cell · 2022Review
- An HNF1α truncation associated with maturity-onset diabetes of the young impairs pancreatic progenitor differentiation by antagonizing HNF1β function.Cell reports · 2022Article
- Human expandable pancreatic progenitor-derived β cells ameliorate diabetes.Science advances · 2022Article
- Leveraging the strengths of mice, human stem cells, and organoids to model pancreas development and diabetes.Frontiers in endocrinology · 2022Review
- Interactions between islets and regulatory immune cells in health and type 1 diabetes.Diabetologia · 2021Review
- CD81 marks immature and dedifferentiated pancreatic β-cells.Molecular metabolism · 2021Article
- A 3D system to model human pancreas development and its reference single-cell transcriptome atlas identify signaling pathways required for progenitor expansion.Nature communications · 2021Article
- Self-organization of organoids from endoderm-derived cells.Journal of molecular medicine (Berlin, Germany) · 2021Review
- Endocrine Pancreas Development and Dysfunction Through the Lens of Single-Cell RNA-Sequencing.Frontiers in cell and developmental biology · 2021Review
- Three-Dimensional Culture of Ameloblast-Originated HAT-7 Cells for Functional Modeling of Defective Tooth Enamel Formation.Frontiers in pharmacology · 2021Article
- Shaping Pancreatic β-Cell Differentiation and Functioning: The Influence of Mechanotransduction.Cells · 2020Review
Corrections and comments
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Authors and funding
7 authors at 3 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
objectiveTranslation of basic research from bench-to-bedside relies on a better understanding of similarities and differences between mouse and human cell biology, tissue formation, and organogenesis. Thus, establishing ex vivo modeling systems of mouse and human pancreas development will help not only to understand evolutionary conserved mechanisms of differentiation and morphogenesis but also to understand pathomechanisms of disease and design strategies for tissue engineering.
methodsHere, we established a simple and reproducible Matrigel-based three-dimensional (3D) cyst culture model system of mouse and human pancreatic progenitors (PPs) to study pancreatic epithelialization and endocrinogenesis ex vivo. In addition, we reanalyzed previously reported single-cell RNA sequencing (scRNA-seq) of mouse and human pancreatic lineages to obtain a comprehensive picture of differential expression of key transcription factors (TFs), cell-cell adhesion molecules and cell polarity components in PPs during endocrinogenesis.
resultsWe generated mouse and human polarized pancreatic epithelial cysts derived from PPs. This system allowed to monitor establishment of pancreatic epithelial polarity and lumen formation in cellular and sub-cellular resolution in a dynamic time-resolved fashion. Furthermore, both mouse and human pancreatic cysts were able to differentiate towards the endocrine fate. This differentiation system together with scRNA-seq analysis revealed how apical-basal polarity and tight and adherens junctions change during endocrine differentiation.
conclusionsWe have established a simple 3D pancreatic cyst culture system that allows to tempo-spatial resolve cellular and subcellular processes on the mechanistical level, which is otherwise not possible in vivo.
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