ReviewMetabolites2019
Experimental Design and Sample Preparation in Forest Tree Metabolomics.
Review in Metabolites, 2019. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 15 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
15 citing papers in PubMed.
- Analyzing Plant Low-Molecular-Weight Polar Metabolites: A GC-MS Approach.Plants (Basel, Switzerland) · 2026Review
- How to Process Samples for Metabolomics in Ecology: A Comparative Study of Preanalytical Storage Methods.Journal of separation science · 2025Article
- Metabolic profiling and antibacterial activity of tree wood extracts obtained under variable extraction conditions.Metabolomics : Official journal of the Metabolomic Society · 2024Article
- A delayed response in phytohormone signaling and production contributes to pine susceptibility to Fusarium circinatum.BMC plant biology · 2024Article
- Plant Metabolomics: An Overview of the Role of Primary and Secondary Metabolites against Different Environmental Stress Factors.Life (Basel, Switzerland) · 2023Review
- Multiomics Molecular Research into the Recalcitrant and OrphanInternational journal of molecular sciences · 2022Review
- Bacterial Metabolomics: Sample Preparation Methods.Biochemistry research international · 2022Review
- Targeted Metabolomics to Assess Exposure to Environmental Chemicals of Concern in Japanese Quail at Two Life Stages.Metabolites · 2021Article
- Kiwifruit Metabolomics-An Investigation of within Orchard Metabolite Variability of Two Cultivars ofMetabolites · 2021Article
- Plant Responses to Abiotic Stresses and Rhizobacterial Biostimulants: Metabolomics and Epigenetics Perspectives.Metabolites · 2021Review
- Metabolomics-Guided Elucidation of Plant Abiotic Stress Responses in the 4IR Era: An Overview.Metabolites · 2021Review
- Approaches in metabolomics for regulatory toxicology applications.The Analyst · 2021Review
- The Effects of Sampling and Storage Conditions on the Metabolite Profile of the Marine SpongeFrontiers in chemistry · 2021Article
- Biostimulants for Plant Growth and Mitigation of Abiotic Stresses: A Metabolomics Perspective.Metabolites · 2020Review
- NMR Metabolomics Applied on the Discrimination of Variables Influencing Tomato (Molecules (Basel, Switzerland) · 2020Review
Corrections and comments
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Authors and funding
3 authors.
Funding
Abstract
Appropriate experimental design and sample preparation are key steps in metabolomics experiments, highly influencing the biological interpretation of the results. The sample preparation workflow for plant metabolomics studies includes several steps before metabolite extraction and analysis. These include the optimization of laboratory procedures, which should be optimized for different plants and tissues. This is particularly the case for trees, whose tissues are complex matrices to work with due to the presence of several interferents, such as oleoresins, cellulose. A good experimental design, tree tissue harvest conditions, and sample preparation are crucial to ensure consistency and reproducibility of the metadata among datasets. In this review, we discuss the main challenges when setting up a forest tree metabolomics experiment for mass spectrometry (MS)-based analysis covering all technical aspects from the biological question formulation and experimental design to sample processing and metabolite extraction and data acquisition. We also highlight the importance of forest tree metadata standardization in metabolomics studies.
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What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.