ArticleBiosensors2019
Characterization of an Aptamer Directed against 25-Hydroxyvitamin D for the Development of a Competitive Aptamer-Based Assay.
Article in Biosensors, 2019. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 12 papers.
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Who cites it
12 citing papers in PubMed.
- Review
- Functionalized Quantum Dot-Based Portable Sensor for Dual Detection of Stress Biomarkers in Blood Serum and Saliva.Journal of biophotonics · 2026Article
- Nanoengineered Calcium Receptors Coupled with Microscale Thermophoresis Enable Sensitive, Low-Volume Quantification of Calcium Ions in Complex Biological Fluids.ACS sensors · 2025Article
- Sensitive and Facile Detection of Vitamin D Based on Fluorescent Labeled Aptamer Probe and Exonuclease I-Assisted Signal Amplification.Journal of fluorescence · 2025Article
- Fabrication of a low-cost benchtop optical imager for quantum dot microarray-based stress biomarker detection.Biomedical optics express · 2024Article
- An AuNPs-based electrochemical aptasensor for the detection of 25-hydroxy vitamin DAnalytical sciences : the international journal of the Japan Society for Analytical Chemistry · 2024Article
- Development of silver nanoparticles and aptamer conjugated biosensor for rapid detection of3 Biotech · 2023Article
- Decentralized vitamin C & D dual biosensor chip: Toward personalized immune system support.Biosensors & bioelectronics · 2021Article
- Novel DNA Aptameric Sensors to Detect the Toxic Insecticide Fenitrothion.International journal of molecular sciences · 2021Article
- Recent Advancements in Aptamer-Based Surface Plasmon Resonance Biosensing Strategies.Biosensors · 2021Review
- All-Optical Planar Polymer Waveguide-Based Biosensor Chip Designed for Smartphone-Assisted Detection of Vitamin D.Sensors (Basel, Switzerland) · 2020Article
- Aptasensors for Point-of-Care Detection of Small Molecules.Biosensors · 2020Review
Corrections and comments
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Authors and funding
5 authors.
Funding
Abstract
Detection of the small molecule 25-hydroxyvitamin D (25(OH)D) as the most relevant marker for vitamin D supply suffers from a high variability of results using the current detection methods, such as high-performance liquid chromatography (HPLC) and immunoassays. A new detection approach using a highly specific aptamer directed against 25(OH)D was established in this study based on the target-induced dissociation (TID) sensing approach. In this work, the aptamer was investigated regarding its structural properties as well as its binding affinity by using microscale thermophoresis (MST). Moreover, complementary oligonucleotides were designed based on the aptamer structure and were evaluated in MST experiments. Binding experiments of immobilized aptamers were conducted in microarray experiments. It could be shown that the aptamer exhibited the usual B-DNA structure and did not form any G-quadruplexes. The design of complementary oligonucleotides for the TID assay identified a putative 25(OH)D binding site within the aptamer. The limit of detection of the established competitive assay was determined to be 5.4 nM, which sets the stage for the development of a biosensor system.
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