Evidence map›Paper›PMID 31765994›Full record

ArticleiScience2019

All-Optical Volumetric Physiology for Connectomics in Dense Neuronal Structures.

Chiao Huang, Chu-Yi Tai, Kai-Ping Yang, Wei-Kun Chang, Kuo-Jen Hsu, Ching-Chun Hsiao, Shun-Chi Wu, Yen-Yin Lin, Ann-Shyn Chiang, Shi-Wei Chu

Abstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in iScience, 2019. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 4 papers.

0numbers the graph read from it
0cells of the map it votes in
4citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

4 citing papers in PubMed.

  1. Article
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4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

10 authors.

Chiao HuangDepartment of Physics, National Taiwan University, 1, Sec 4, Roosevelt Road, Taipei 10617, Taiwan.
Chu-Yi TaiInstitute of Biotechnology, National Tsing Hua University, 101, Sec 2, Guangfu Road, Hsinchu 30013, Taiwan.
Kai-Ping YangDepartment of Physics, National Taiwan University, 1, Sec 4, Roosevelt Road, Taipei 10617, Taiwan.
Wei-Kun ChangBrain Research Center, National Tsing Hua University, 101, Sec 2, Guangfu Road, Hsinchu 30013, Taiwan.
Kuo-Jen HsuDepartment of Physics, National Taiwan University, 1, Sec 4, Roosevelt Road, Taipei 10617, Taiwan; Brain Research Center, National Tsing Hua University, 101, Sec 2, Guangfu Road, Hsinchu 30013, Taiwan.
Ching-Chun HsiaoDepartment of Engineering and System Science, National Tsing Hua University, 101, Sec 2, Guangfu Road, Hsinchu 30013, Taiwan.
Shun-Chi WuDepartment of Engineering and System Science, National Tsing Hua University, 101, Sec 2, Guangfu Road, Hsinchu 30013, Taiwan.
Yen-Yin LinBrain Research Center, National Tsing Hua University, 101, Sec 2, Guangfu Road, Hsinchu 30013, Taiwan. Electronic address: yylin@life.nthu.edu.tw.
Ann-Shyn ChiangInstitute of Biotechnology, National Tsing Hua University, 101, Sec 2, Guangfu Road, Hsinchu 30013, Taiwan; Brain Research Center, National Tsing Hua University, 101, Sec 2, Guangfu Road, Hsinchu 30013, Taiwan; Institute of Systems Neuroscience, National Tsing Hua University, 101, Sec 2, Guangfu Road, Hsinchu 30013, Taiwan; Department of Biomedical Science and Environmental Biology, Kaohsiung Medical University, Kaohsiung 80780, Taiwan; Graduate Institute of Clinical Medical Science, China Medical University, Taichung 40402, Taiwan; Institute of Molecular and Genomic Medicine, National Health Research Institutes, Zhunan, Miaoli 35053, Taiwan; Kavli Institute for Brain and Mind, University of California, San Diego, CA 92161, USA. Electronic address: aschiang@life.nthu.edu.tw.
Shi-Wei ChuDepartment of Physics, National Taiwan University, 1, Sec 4, Roosevelt Road, Taipei 10617, Taiwan; Molecular Imaging Center, National Taiwan University, 1, Sec 4, Roosevelt Road, Taipei 10617, Taiwan. Electronic address: swchu@phys.ntu.edu.tw.

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

All-optical physiology (AOP) manipulates and reports neuronal activities with light, allowing for interrogation of neuronal functional connections with high spatiotemporal resolution. However, contemporary high-speed AOP platforms are limited to single-depth or discrete multi-plane recordings that are not suitable for studying functional connections among densely packed small neurons, such as neurons in Drosophila brains. Here, we constructed a 3D AOP platform by incorporating single-photon point stimulation and two-photon high-speed volumetric recordings with a tunable acoustic gradient-index (TAG) lens. We demonstrated the platform effectiveness by studying the anterior visual pathway (AVP) of Drosophila. We achieved functional observation of spatiotemporal coding and the strengths of calcium-sensitive connections between anterior optic tubercle (AOTU) sub-compartments and >70 tightly assembled 2-μm bulb (BU) microglomeruli in 3D coordinates with a single trial. Our work aids the establishment of in vivo 3D functional connectomes in neuron-dense brain areas.

Indexed as

Biological Sciences Research MethodologiesBiological Sciences ToolsNeuroscienceTechniques in Neuroscience

Identifiers

PMID31765994
PMCPMC6883334

What OpenQuestion holds

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Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.