Evidence map›Paper›PMID 31765730›Full record

ReviewNeuroscience letters2020

Super-resolution microscopy for analyzing neuromuscular junctions and synapses.

Yomna Badawi, Hiroshi Nishimune

Open access · greenAbstract readReview
In one paragraph

Review in Neuroscience letters, 2020. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 13 papers.

0numbers the graph read from it
0cells of the map it votes in
13citing papers in PubMed
2.7field-weighted citation impact, top 9% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

13 citing papers in PubMed, 21 citations in OpenAlex.

  1. Junctions in Jeopardy: the neuromuscular junction is a selective pathological target in Charcot-Marie-Tooth disease.Mammalian genome : official journal of the International Mammalian Genome Society · 2026
    Review
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  4. Advances in Cellular and Molecular Biology Assays: A Review of Gold Standard Methods.International journal of innovative science and research technology · 2025
    Article
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4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

2 authors at 1 institution in 1 country.

Yomna BadawiDepartment of Anatomy and Cell Biology, University of Kansas School of Medicine, Kansas City, KS, 66160, USA.
Hiroshi NishimuneDepartment of Anatomy and Cell Biology, University of Kansas School of Medicine, Kansas City, KS, 66160, USA. Electronic address: hnishimune@kumc.edu.
University of Kansas · US

Funding

Exercise-activated mechanisms reduce degeneration of aged neuromuscular junctionsR01AG051470 · NIA · UNIVERSITY OF KANSAS MEDICAL CENTER · PI MERINEY, STEPHEN D, NISHIMUNE, HIROSHI · 2015 to 2019
$1.9M
Super resolution microscope for the imaging core facilityS10OD023625 · OD · UNIVERSITY OF KANSAS MEDICAL CENTER · PI NISHIMUNE, HIROSHI · 2019 to 2019
$987k
NIA NIH HHS R01 AG051470NIH HHS S10 OD023625
6 · The paper itself

Abstract

Super-resolution microscopy techniques offer subdiffraction limited resolution that is two- to ten-fold improved compared to that offered by conventional confocal microscopy. This breakthrough in resolution for light microscopy has contributed to new findings in neuroscience and synapse biology. This review will focus on the Structured Illumination Microscopy (SIM), Stimulated emission depletion (STED) microscopy, and Stochastic optical reconstruction microscopy (STORM) / Single molecule localization microscopy (SMLM) techniques and compare them for the better understanding of their differences and their suitability for the analysis of synapse biology. In addition, we will discuss a few practical aspects of these microscopic techniques, including resolution, image acquisition speed, multicolor capability, and other advantages and disadvantages. Tips for the improvement of microscopy will be introduced; for example, information resources for recommended dyes, the limitations of multicolor analysis, and capabilities for live imaging. In addition, we will summarize how super-resolution microscopy has been used for analyses of neuromuscular junctions and synapses.

Indexed as

SynapsesAnimalsHumansMicroscopy, FluorescenceNeuromuscular JunctionNeuromuscular junctionSIMSTEDSTORMSuper-resolution microscopySynapse

Identifiers

PMID31765730
PMCPMC6937598
OpenAlexW2991563793

What OpenQuestion holds

Textmetadata
LicenceTDM
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.