ArticlePloS one2019
Genome-wide histone modification profiling of inner cell mass and trophectoderm of bovine blastocysts by RAT-ChIP.
Article in PloS one, 2019. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 8 papers.
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Who cites it
8 citing papers in PubMed.
- Molecular cloning of PRD-like homeobox genes expressed in bovine oocytes and early IVF embryos.BMC genomics · 2024Article
- Molecular and cellular programs underlying the development of bovine pre-implantation embryos.Reproduction, fertility, and development · 2023Article
- The establishment of CDK9/RNA PolII/H3K4me3/DNA methylation feedback promotes HOTAIR expression by RNA elongation enhancement in cancer.Molecular therapy : the journal of the American Society of Gene Therapy · 2022Article
- RGD v2.0: a major update of the ruminant functional and evolutionary genomics database.Nucleic acids research · 2022Article
- Comparative analysis of histone H3K4me3 modifications between blastocysts and somatic tissues in cattle.Scientific reports · 2021Article
- The Epigenetics of Gametes and Early Embryos and Potential Long-Range Consequences in Livestock Species-Filling in the Picture With Epigenomic Analyses.Frontiers in genetics · 2021Review
- Efficient low-cost chromatin profiling with CUT&Tag.Nature protocols · 2020Article
- Current status of genome-wide epigenetic profiling of mammalian preimplantation embryos.Reproductive medicine and biologyReview
Corrections and comments
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Authors and funding
9 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Chromatin immunoprecipitation coupled with next-generation sequencing (ChIP-seq) has revolutionized our understanding of chromatin-related biological processes. The method, however, requires thousands of cells and has therefore limited applications in situations where cell numbers are limited. Here we describe a novel method called Restriction Assisted Tagmentation Chromatin Immunoprecipitation (RAT-ChIP) that enables global histone modification profiling from as few as 100 cells. The method is simple, cost-effective and takes a single day to complete. We demonstrate the sensitivity of the method by deriving the first genome-wide maps of histone H3K4me3 and H3K27me3 modifications of inner cell mass and trophectoderm of bovine blastocyst stage embryos.
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