Evidence map›Paper›PMID 31763120›Full record

Article3 Biotech2019

Progress toward sourcing plants for new bioconjugation tools: a screening evaluation of a model peptide ligase using a synthetic precursor.

Tunjung Mahatmanto, Isyatul Azizah, Alex Buchberger, Nicholas Stephanopoulos

Abstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in 3 Biotech, 2019. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

0numbers the graph read from it
0cells of the map it votes in
0citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

0 citing papers in PubMed.

No citing paper in PubMed yet.

4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

4 authors.

Tunjung Mahatmanto1Department of Agricultural Product Technology, Faculty of Agricultural Technology, Universitas Brawijaya, Malang, East Java Indonesia.ORCID 0000-0002-3708-045X
Isyatul Azizah2Center for Molecular Design and Biomimetics at the Biodesign Institute, Arizona State University, Tempe, AZ USA.
Alex Buchberger2Center for Molecular Design and Biomimetics at the Biodesign Institute, Arizona State University, Tempe, AZ USA.
Nicholas Stephanopoulos2Center for Molecular Design and Biomimetics at the Biodesign Institute, Arizona State University, Tempe, AZ USA.ORCID 0000-0001-7859-410X

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

In the present study, leaves from 39 phylogenetically distant plant species were sampled and screened for asparaginyl endopeptidase ligase activity using mass spectrometry to test the generality of peptide ligases in plants. A modified version of the sunflower trypsin inhibitor-1 precursor was used as the substrate for reactions with leaf crude extracts and protein fractions. Masses consistent with products of asparaginyl endopeptidase activities that cleave and ligate the substrate into cyclic peptide following the reactions were detected in 8 plants:

Indexed as

Asparaginyl endopeptidaseBioconjugation toolCyclic peptidePlant peptide ligaseProtein modification

Identifiers

PMID31763120
PMCPMC6842375

What OpenQuestion holds

Textmetadata
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.