ArticleAnalytical methods : advancing methods and applications2019
Measurement of methylated metabolites using Liquid Chromatography-Mass Spectrometry and its biological application.
Article in Analytical methods : advancing methods and applications, 2019. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 7 papers.
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Who cites it
7 citing papers in PubMed, 17 citations in OpenAlex.
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- Mitochondrial reprogramming by activating OXPHOS via glutamine metabolism in African American patients with bladder cancer.JCI insight · 2024Article
- Immune gene patterns and characterization of the tumor immune microenvironment associated with cancer immunotherapy efficacy.Heliyon · 2023Article
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- Gut microbiota-derived short-chain fatty acids protect against the progression of endometriosis.Life science alliance · 2021Article
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- Multiblock metabolomics: An approach to elucidate whole-body metabolism with multiblock principal component analysis.Computational and structural biotechnology journal · 2021Article
Corrections and comments
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Authors and funding
10 authors at 1 institution in 1 country.
Funding
Abstract
Methylation aberrations play an important role in many metabolic disorders including cancer. Methylated metabolites are direct indicators of metabolic aberrations, and currently, there is no Liquid chromatography - Mass spectrometry (LC-MS) based method available to cover all classes of methylated metabolites at low detection limits. In this study, we have developed a method for the detection of methylated metabolites, and it's biological application. In this approach, we used a HILIC based HPLC with MS to measure methylated organic acids, amino acids, and nucleotides. These metabolites were separated from each other by their hydrophobic interactions and analyzed by targeted metabolomics of single reaction monitoring by positive and negative mode of electrospray ionization. These metabolites were quantified, and the interday reproducibility was <10% relative standard deviation. Furthermore, by applying this method, we identified high levels of methylated metabolites in bladder cancer cell lines compared to benign cells.
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Registered trials
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