Evidence map›Paper›PMID 31762614›Full record

ArticleSaudi journal of biological sciences2019

Physical and chemical mutagens improved

Asim Mehmood, Umal Baneen, Ahmad Zaheer, Muhammad Wasim Sajid, Abrar Hussain, Shahzad Saleem, Qudsia Yousafi, Hamid Rashid, Hassan Riaz, Awais Ihsan and 8 more

Open access · hybridAbstract read
In one paragraph

Article in Saudi journal of biological sciences, 2019. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 1 paper.

0numbers the graph read from it
0cells of the map it votes in
1citing papers in PubMed
1.5field-weighted citation impact, top 14% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

1 citing paper in PubMed, 10 citations in OpenAlex.

  1. Review
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

18 authors at 5 institutions in 2 countries.

Asim MehmoodDepartment of Biosciences, COMSATS University Islamabad, Sahiwal, Pakistan.
Umal BaneenDepartment of Biosciences, COMSATS University Islamabad, Sahiwal, Pakistan.
Ahmad ZaheerInstitute of Molecular Biology and Biotechnology, The University of Lahore, Defence Road, Lahore, Pakistan.
Muhammad Wasim SajidDepartment of Biosciences, COMSATS University Islamabad, Sahiwal, Pakistan.
Abrar HussainDepartment of Biosciences, COMSATS University Islamabad, Sahiwal, Pakistan.
Shahzad SaleemDepartment of Biosciences, COMSATS University Islamabad, Sahiwal, Pakistan.
Qudsia YousafiDepartment of Biosciences, COMSATS University Islamabad, Sahiwal, Pakistan.
Hamid RashidDepartment of Biosciences, COMSATS University Islamabad, Sahiwal, Pakistan.
Hassan RiazDepartment of Biosciences, COMSATS University Islamabad, Sahiwal, Pakistan.
Awais IhsanDepartment of Biosciences, COMSATS University Islamabad, Sahiwal, Pakistan.
Farrukh JamilDepartment of Biosciences, COMSATS University Islamabad, Sahiwal, Pakistan.
Yasar SajjadDepartment of Biotechnology, COMSATS University Islamabad, Abbottabad Campus, Pakistan.
Nageena ZahidInstitute of Molecular Biology and Biotechnology (IMBB), Bahauddin Zakariya University, Multan, Pakistan.
Muhammad Shahzad AnjamInstitute of Molecular Biology and Biotechnology (IMBB), Bahauddin Zakariya University, Multan, Pakistan.
Muhammad ArshadDepartment of Biotechnology, University of Okara, Pakistan.
Zeenat MirzaKing Fahd Medical Research Center, King Abdulaziz University, Jeddah, Saudi Arabia.
Sajjad KarimCenter of Excellence in Genomic Medicine Research (CEGMR), King Abdulaziz University, Jeddah, Saudi Arabia.
Mahmood RasoolCenter of Excellence in Genomic Medicine Research (CEGMR), King Abdulaziz University, Jeddah, Saudi Arabia.
COMSATS University Islamabad · PKKing Abdulaziz University · SABahauddin Zakariya University · PKUniversity of Lahore · PKUniversity of Okara · PK

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

objectivePhosphorous is an essential micronutrient of plants and involved in critical biological functions. In nature, phosphorous is mostly present in immobilized inorganic mineral and in the fixed organic form including phytic acid and phosphoesteric compounds. However, the bioavailability of bound phosphorous could be enhanced by the use of phosphate solubilizing microorganisms such as bacteria and fungi. The phytases are widespread in an environment and have been isolated from different sources comprising bacteria and fungi. METHODOLOGY: In current studies, we show the successful use of gamma rays and EMS (Ethyl Methane Sulphonate) mutagenesis for enhanced activity of phytases in a fungal strain

resultsWe report an improved strain ST2 that could produce a clear halo zone around the colony, up to 24 mm. The maximum enzymatic activity was found of 382 U/mL on pH 5.5. However, the phytase activity was improved to 387 U/ml at 45 °C. We also report that the mutants produced through EMS showed the greater potential for phytase production.

conclusionThe current study highlights the potential of EMS mutagenesis for strain improvement over physical mutagens.

Indexed as

EMS mutagenesisFungusGamma raysPhosphate solubilizer

Identifiers

PMID31762614
PMCPMC6864213
OpenAlexW2960256984

What OpenQuestion holds

Textmetadata
LicenceCC BY-NC-ND
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.