ArticleIndian journal of microbiology2019
Comparison of DNA Extraction Methods for Optimal Recovery of Metagenomic DNA from Human and Environmental Samples.
Article in Indian journal of microbiology, 2019. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 5 papers.
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Who cites it
5 citing papers in PubMed.
- Protocol for efficient recovery of high-quality DNA from microbiome of marine invertebrates.Journal of microbiology (Seoul, Korea) · 2025Article
- Laboratory and In-Field Metagenomics for Environmental Monitoring.Methods in molecular biology (Clifton, N.J.) · 2025Article
- Improvement and Validation of a Genomic DNA Extraction Method for Human Breastmilk.Methods and protocols · 2023Article
- Analysis of microbiota-host communication mediated by butyrate in Atlantic salmon.Computational and structural biotechnology journal · 2023Article
- Diet, Gut Microbiota and COVID-19.Indian journal of microbiology · 2020Review
Corrections and comments
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Authors and funding
10 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Metagenomics is the study of gene pool of an entire community in a particular niche. This provides valuable information about the functionality of host-microbe interaction in a biological ecosystem. Efficient metagenomic DNA extraction is a critical pre-requisite for a successful sequencing run in a metagenomic study. Although isolation of human stool metagenomic DNA is fairly standardized, the same protocol does not work as efficiently in fecal DNA from other organisms. In this study, we report a comparison of manual and commercial DNA extraction methods for diverse samples such as human stool, fish gut and soil. Fishes are known to have variable microbial diversity based on their food habits, so the study included two different varieties of fishes. A modified protocol for effective isolation of metagenomic DNA from human milk samples is also reported, highlighting critical precautions. Recent studies have emphasized the importance of studying functionality of human milk metagenome to understand its influence on infants' health. While manual method works well with most samples and therefore can be a method of choice for testing new samples, broad-range commercial kit offers advantage of high purity and quality. DNA extraction of different samples would go a long way in unraveling the unexplored association between microbes and host in a biological system.
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Registered trials
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