Evidence map›Paper›PMID 31758676›Full record

ArticleJournal of biomedical optics2019

Bacteriophage-mediated identification of bacteria using photoacoustic flow cytometry.

Robert H Edgar, Justin Cook, Cierra Noel, Austin Minard, Andrea Sajewski, Matthew Fitzpatrick, Rachel Fernandez, John D Hempel, John A Kellum, John A Viator

Abstract read
In one paragraph

Article in Journal of biomedical optics, 2019. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 6 papers.

0numbers the graph read from it
0cells of the map it votes in
6citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

6 citing papers in PubMed.

  1. Article
  2. Article
  3. The sound of blood: photoacoustic imaging in blood analysis.Medicine in novel technology and devices · 2023
    Article
  4. Article
  5. Article
  6. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

10 authors.

Robert H EdgarUniversity of Pittsburgh, Swanson School of Engineering, Department of Bioengineering, Pittsburgh, P, United States.
Justin CookDuquesne University, Pittsburgh, Pennsylvania, United States.
Cierra NoelDuquesne University, Pittsburgh, Pennsylvania, United States.
Austin MinardDuquesne University, Pittsburgh, Pennsylvania, United States.
Andrea SajewskiDuquesne University, Pittsburgh, Pennsylvania, United States.
Matthew FitzpatrickDuquesne University, Pittsburgh, Pennsylvania, United States.
Rachel FernandezDuquesne University, Pittsburgh, Pennsylvania, United States.
John D HempelDuquesne University, Pittsburgh, Pennsylvania, United States.
John A KellumUniversity of Pittsburgh, Center for Critical Care Nephrology, Department of Critical Care Medicine,, United States.
John A ViatorUniversity of Pittsburgh, Swanson School of Engineering, Department of Bioengineering, Pittsburgh, P, United States.

Funding

Photoacoustic detection, capture, and analysis of circulating melanoma cellsR01CA182840 · NCI · DUQUESNE UNIVERSITY · PI VIATOR, JOHN A. · 2014 to 2018
$1.4M
NCI NIH HHS R01CA161367NCI NIH HHS R01 CA182840
6 · The paper itself

Abstract

Infection with resistant bacteria has become an ever increasing problem in modern medical practice. Currently, broad spectrum antibiotics are prescribed until bacteria can be identified through blood cultures, a process that can take two to three days and is unable to provide quantitative information. To detect and quantify bacteria rapidly in blood samples, we designed a method using labeled bacteriophage in conjunction with photoacoustic flow cytometry (PAFC). PAFC is the generation of ultrasonic waves created by the absorption of laser light in particles under flow. Bacteriophage is a virus that infects bacteria and possesses the ability to discriminate bacterial surface antigens, allowing the bacteriophage to bind only to their target bacteria. Bacteria can be tagged with dyed phage and processed through a photoacoustic flow cytometer where they are detected by the acoustic response. We demonstrate that

Indexed as

BacteriophagesColoring AgentsEpitopesEscherichia coliFlow CytometryLasersPhotoacoustic TechniquesSalmonellaSignal Processing, Computer-AssistedUltrasonicsColoring AgentsEpitopesbacteriophagediagnosticsoptoacoustics

Identifiers

PMID31758676
PMCPMC6874036

What OpenQuestion holds

Textmetadata
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.