Evidence map›Paper›PMID 31758428›Full record

ArticleMarine biotechnology (New York, N.Y.)2019

HaCeD-Seq: a Novel Method for Reliable and Easy Estimation About the Fish Population Using Haplotype Count from eDNA.

Kazutoshi Yoshitake, Tatsuki Yoshinaga, Chikaya Tanaka, Nanami Mizusawa, Md Shaheed Reza, Atsumi Tsujimoto, Takanori Kobayashi, Shugo Watabe

Abstract read
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In one paragraph

Article in Marine biotechnology (New York, N.Y.), 2019. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 6 papers.

0numbers the graph read from it
0cells of the map it votes in
6citing papers in PubMed
1.2field-weighted citation impact, top 20% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

6 citing papers in PubMed, 28 citations in OpenAlex.

  1. Article
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  5. Estimating number of European eel (Ecology and evolution · 2023
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4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

8 authors at 2 institutions in 1 country.

Kazutoshi YoshitakeSchool of Marine Biosciences, Kitasato University, Sagamihara, Kanagawa, Japan.
Tatsuki YoshinagaSchool of Marine Biosciences, Kitasato University, Sagamihara, Kanagawa, Japan.
Chikaya TanakaSchool of Marine Biosciences, Kitasato University, Sagamihara, Kanagawa, Japan.
Nanami MizusawaSchool of Marine Biosciences, Kitasato University, Sagamihara, Kanagawa, Japan.
Md Shaheed RezaSchool of Marine Biosciences, Kitasato University, Sagamihara, Kanagawa, Japan.
Atsumi TsujimotoJapan Software Management Co., Ltd., Kinko-cho, Yokohama, Kanagawa, Japan.
Takanori KobayashiSchool of Marine Biosciences, Kitasato University, Sagamihara, Kanagawa, Japan.
Shugo WatabeSchool of Marine Biosciences, Kitasato University, Sagamihara, Kanagawa, Japan. swatabe@kitasato-u.ac.jp.ORCID http://orcid.org/0000-0002-0037-7778
Kitasato University · JPJapan Fisheries Research and Education Agency · JP

Funding

Fisheries Agency,Council of Agriculture No number
6 · The paper itself

Abstract

It is common to count the numbers of specified fish in the field after speciation of captured fish according to their morphology and to subject these counts to appropriate statistical analyses. In recent years, a non-invasive method to estimate the abundance of a particular fish species using environmental DNA (eDNA) has been developed. However, it is still difficult to determine accurate numbers of fish species using such method. We predict that the estimation of individuals of certain fish species in the field is more accurate and easier by using haplotypes of DNA in the fast evolutionary region. Therefore, we focused on the regulatory region (D-loop) in mitochondrial DNA, which is known to have a high genetic variation at the intraspecific level of the targeting eel. We investigated haplotype diversity in eel at first and then determined the number of D-loop haplotypes contained in their exfoliated cells in breeding water. Finally, we developed a novel analytical method, HaCeD-Seq, to estimate the number of individuals based on the abovementioned data.

Indexed as

HaplotypesAnguillaAnimalsAquacultureDNA, MitochondrialEnvironmental MonitoringWaterDNA, MitochondrialWaterAnalytical pipelineD-loopeDNAEelFish populationHaplotype diversity

Identifiers

PMID31758428
OpenAlexW2990204952

What OpenQuestion holds

Textmetadata
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.