ArticleMikrochimica acta2019
Immunoliposome-based fluorometric patulin assay by using immunomagnetic nanoparticles.
Article in Mikrochimica acta, 2019. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 2 papers.
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Who cites it
2 citing papers in PubMed.
- Evaluation of the Immune Response of Patulin by Proteomics.Biosensors · 2024Article
- Carbon nanotube-mediated antibody-free suspension array for determination of typical endocrine-disrupting chemicals.Mikrochimica acta · 2020Article
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3 authors.
Funding
Abstract
A fluorometric immunoassay is described for the determination of patulin, a highly toxic fungal metabolite. A rabbit anti-patulin-bovine serum albumin (BSA) IgG conjugate was prepared and used to compose immunoliposomes and immunomagnetic nanoparticles. The immunomagnetic nanoparticles are then added to the sample to form the patulin-antibody composites which can be magnetically separated. The immunoliposomes are then added to form a sandwich. After magnetic separation of the composites and adding n-octyl-β-D-glucopyranoside, the fluorophore sulforhodamine B (SRB) is released. Its fluorescence intensity was then measured at excitation/emission wavelengths of 550/585 nm. The immunoliposome-based immunomagnetic nanoparticle assay can detect 8 μg L
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