ReviewCellular and molecular life sciences : CMLS2020
The common message of constraint-based optimization approaches: overflow metabolism is caused by two growth-limiting constraints.
Review in Cellular and molecular life sciences : CMLS, 2020. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 43 papers.
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43 citing papers in PubMed.
- RNA thermometers as temperature-dependent tools for enhanced recombinant protein production in precision fermentation.Microbial cell factories · 2026Article
- Thermo-flux: generation and analysis of thermodynamic-stoichiometric metabolic network models.Molecular systems biology · 2026Article
- Acetate-Linked Energy Metabolism as a Determinant of Early Haemophilus influenzae Infection Fitness.MicrobiologyOpen · 2026Article
- On the conditions for shifts in metabolic strategies.PLoS computational biology · 2026Review
- Mathematical Modelling and Intuition in Microbiology: A Perspective.Environmental microbiology · 2026Review
- Potent Probiotic YeastACS omega · 2026Article
- Overflow metabolism in bacterial, yeast, and mammalian cells: different names, same game.Molecular systems biology · 2025Review
- Global constraint principle for microbial growth laws.Proceedings of the National Academy of Sciences of the United States of America · 2025Article
- Cross-feeding percolation phase transitions of intercellular metabolic networks.Science advances · 2025Article
- Gut microbe-derived lactic acid optimizes host energy metabolism during starvation.bioRxiv : the preprint server for biology · 2025Article
- Controlled burn: interconnections between energy-spilling pathways and metabolic signaling in bacteria.Journal of bacteriology · 2025Review
- When is microbial cross-feeding evolutionarily stable?bioRxiv : the preprint server for biology · 2025Article
- COBREXA 2: tidy and scalable construction of complex metabolic models.Bioinformatics (Oxford, England) · 2025Article
- Sensitivities in protein allocation models reveal distribution of metabolic capacity and flux control.Bioinformatics (Oxford, England) · 2024Article
- Multiscale modelling of bioprocess dynamics and cellular growth.Microbial cell factories · 2024Article
- Free-energy transduction mechanisms shape the flux space of metabolic networks.Biophysical journal · 2024Article
- A hierarchy of metabolite exchanges in metabolic models of microbial species and communities.PLoS computational biology · 2024Article
- Emergence of Orchestrated and Dynamic Metabolism ofACS synthetic biology · 2024Article
- Microbial Pathway Thermodynamics: Stoichiometric Models Unveil Anabolic and Catabolic Processes.Life (Basel, Switzerland) · 2024Article
- Development and applications of metabolic models in plant multi-omics research.Frontiers in plant science · 2024Review
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6 authors.
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Abstract
Living cells can express different metabolic pathways that support growth. The criteria that determine which pathways are selected in which environment remain unclear. One recurrent selection is overflow metabolism: the simultaneous usage of an ATP-efficient and -inefficient pathway, shown for example in Escherichia coli, Saccharomyces cerevisiae and cancer cells. Many models, based on different assumptions, can reproduce this observation. Therefore, they provide no conclusive evidence which mechanism is causing overflow metabolism. We compare the mathematical structure of these models. Although ranging from flux balance analyses to self-fabricating metabolism and expression models, we can rewrite all models into one standard form. We conclude that all models predict overflow metabolism when two, model-specific, growth-limiting constraints are hit. This is consistent with recent theory. Thus, identifying these two constraints is essential for understanding overflow metabolism. We list all imposed constraints by these models, so that they can hopefully be tested in future experiments.
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