ArticleScientific reports2019
Membrane-bound Gaussia luciferase as a tool to track shedding of membrane proteins from the surface of extracellular vesicles.
Article in Scientific reports, 2019. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 15 papers.
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Who cites it
15 citing papers in PubMed.
- Inter-Organ Crosstalk and Regulatory Mechanisms of Skeletal Muscle-Derived Extracellular Vesicles in Systemic Metabolic Homeostasis.Journal of cellular and molecular medicine · 2025Review
- HaloTag display enables quantitative single-particle characterisation and functionalisation of engineered extracellular vesicles.Journal of extracellular vesicles · 2024Article
- Dysregulation of intercellular communication in vitro and in vivo via extracellular vesicles secreted by pancreatic duct adenocarcinoma cells and generated under the influence of the AG9 elastin peptide-conditioned microenvironment.Journal of extracellular biology · 2024Article
- Tracking human neurologic disease status in mouse brain/plasma using reporter-tagged, EV-associated biomarkers.Molecular therapy : the journal of the American Society of Gene Therapy · 2023Article
- Engineered EVs designed to target diseases of the CNS.Journal of controlled release : official journal of the Controlled Release Society · 2023Review
- Using genetically modified extracellular vesicles as a non-invasive strategy to evaluate brain-specific cargo.Biomaterials · 2022Article
- In vivo imaging of EVs in zebrafish: New perspectives from "the waterside".FASEB bioAdvances · 2021Review
- The power of imaging to understand extracellular vesicle biology in vivo.Nature methods · 2021Review
- Article
- Exosome-based liquid biopsies in cancer: opportunities and challenges.Annals of oncology : official journal of the European Society for Medical Oncology · 2021Review
- Development of Extracellular Vesicle Therapeutics: Challenges, Considerations, and Opportunities.Frontiers in cell and developmental biology · 2021Review
- Small but Fierce: Tracking the Role of Extracellular Vesicles in Glioblastoma Progression and Therapeutic Resistance.Advanced biosystems · 2020Review
- Palmitoylated mNeonGreen Protein as a Tool for Visualization and Uptake Studies of Extracellular Vesicles.Membranes · 2020Article
- Article
- RNA delivery by extracellular vesicles in mammalian cells and its applications.Nature reviews. Molecular cell biology · 2020Review
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Authors and funding
19 authors.
Funding
Abstract
Extracellular vesicles (EVs) released by cells play a role in intercellular communication. Reporter and targeting proteins can be modified and exposed on the surface of EVs to investigate their half-life and biodistribution. A characterization of membrane-bound Gaussia luciferase (mbGluc) revealed that its signal was detected also in a form smaller than common EVs (<70 nm). We demonstrated that mbGluc initially exposed on the surface of EVs, likely undergoes proteolytic cleavage and processed fragments of the protein are released into the extracellular space in active form. Based on this observation, we developed a new assay to quantitatively track shedding of membrane proteins from the surface of EVs. We used this assay to show that ectodomain shedding in EVs is continuous and is mediated by specific proteases, e.g. metalloproteinases. Here, we present a novel tool to study membrane protein cleavage and release using both in vitro and in vivo models.
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