Evidence map›Paper›PMID 31756731›Full record

ArticlePediatric research2020

Lyophilization of human amniotic fluid is feasible without affecting biological activity.

John I Coon, Sangeeta Jain, Krishna M Sepuru, Yerin Chung, Krishnan Mohankumar, Krishna Rajarathnam, Sunil K Jain

Open access · bronzeAbstract read
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In one paragraph

Article in Pediatric research, 2020. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 2 papers.

0numbers the graph read from it
0cells of the map it votes in
2citing papers in PubMed
0.3field-weighted citation impact, top 40% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

2 citing papers in PubMed, 2 citations in OpenAlex.

  1. Review
  2. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

7 authors at 1 institution in 1 country.

John I CoonDepartment of Pediatrics, Division of Neonatology, University of Texas Medical Branch, Galveston, TX, USA.
Sangeeta JainDepartment of Obstetrics and Gynecology, Division of Maternal Fetal Medicine, University of Texas Medical Branch, Galveston, TX, USA.
Krishna M SepuruDepartment of Biochemistry & Molecular Biology, University of Texas Medical Branch, Galveston, TX, USA.
Yerin ChungDepartment of Pediatrics, Division of Neonatology, John Hopkins University, Baltimore, MD, USA.
Krishnan MohankumarDepartment of Pediatrics, Division of Neonatology, John Hopkins University, Baltimore, MD, USA.
Krishna RajarathnamDepartment of Biochemistry & Molecular Biology, University of Texas Medical Branch, Galveston, TX, USA.
Sunil K JainDepartment of Pediatrics, Division of Neonatology, University of Texas Medical Branch, Galveston, TX, USA. skjian@utmb.edu.
The University of Texas Medical Branch at Galveston · US

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

backgroundFetal swallowing of human amniotic fluid (hAF) containing trophic factors (TFs) promotes gastrointestinal tract (GIT) development. Preterm birth interrupts hAF swallowing, which may increase the risk of necrotizing enterocolitis (NEC). Postnatally, it is difficult to replicate fetal swallowing of hAF due to volume. We aimed to evaluate whether hAF lyophilization is feasible and its effect on hAF-borne TFs.

methodsWe collected hAF (n = 16) from uncomplicated pregnancies. hAF was divided into three groups: unprocessed control (C), concentration by microfiltration (F), and by dialysis and lyophilization (L). EGF, HGF, GM-CSF, and TGF-α were measured in each group by multiplex assay. Bioavailability of TFs was measured by proliferation and LPS-induced IL-8 production by intestinal epithelial cells FHs74.

resultsAfter dialysis/lyophilization, GM-CSF and TGF-α were preserved with partial loss of EGF and HGF. hAF increased cell proliferation and reduced LPS-induced IL-8 production compared to medium alone. Compared to control, dialysis/lyophilization and filtration of hAF increased FHs74 cell proliferation (p < 0.001) and decreased LPS-induced IL-8 production (p < 0.01).

conclusionsLyophilization and filtration of hAF is feasible with partial loss of TFs but maintains and even improves bioavailability of TFs measured by proliferation and LPS-induced IL-8 production by FHs74.

Indexed as

Freeze DryingAmniotic FluidCell ProliferationCryopreservationDeglutitionEnterocolitis, NecrotizingFemaleGastrointestinal TractGranulocyte-Macrophage Colony-Stimulating FactorHumansInflammationInterleukin-8PregnancyTransforming Growth Factor alphaCXCL8 protein, humanGranulocyte-Macrophage Colony-Stimulating FactorInterleukin-8Transforming Growth Factor alpha

Identifiers

PMID31756731
OpenAlexW2990124438

What OpenQuestion holds

Textmetadata
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.