ArticlePloS one2019
Arabidopsis TRM5 encodes a nuclear-localised bifunctional tRNA guanine and inosine-N1-methyltransferase that is important for growth.
Article in PloS one, 2019. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 17 papers.
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17 citing papers in PubMed, 25 citations in OpenAlex.
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- Genome-Wide Identification and Expression Analysis of TONNEAU1 Recruited Motif (TRM) Gene Family in Tomato.International journal of molecular sciences · 2025Article
- The Causal Association Analysis between Depression and Cerebrospinal Fluid: From the Perspective of Mendelian Randomization.Psychology research and behavior management · 2025Article
- Understanding the triacylglycerol-based carbon anabolic differentiation in Cyperus esculentus and Cyperus rotundus developing tubers via transcriptomic and metabolomic approaches.BMC plant biology · 2024Article
- Identification and validation of two major QTLs for spikelet number per spike in wheat (Frontiers in plant science · 2023Article
- Identification of QTLs for wheat heading time across multiple-environments.TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik · 2022Article
- MicroRNA-resistant alleles ofScience advances · 2022Article
- Epitranscriptomics: An Additional Regulatory Layer in Plants' Development and Stress Response.Plants (Basel, Switzerland) · 2022Review
- Variations in transfer and ribosomal RNA epitranscriptomic status can adapt eukaryote translation to changing physiological and environmental conditions.RNA biology · 2021Review
- The occurrence order and cross-talk of different tRNA modifications.Science China. Life sciences · 2021Review
- Article
- Genetic and Molecular Factors Determining Grain Weight in Rice.Frontiers in plant science · 2021Review
- tRNA Metabolism and Lung Cancer: Beyond Translation.Frontiers in molecular biosciences · 2021Review
- Performance evaluation of lossy quality compression algorithms for RNA-seq data.BMC bioinformatics · 2020Article
- Advanced High-Throughput Root Phenotyping and GWAS Identifies Key Genomic Regions in Cowpea During Vegetative Growth Stage.Physiologia plantarumArticle
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14 authors at 5 institutions in 3 countries.
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No grant is acknowledged in the PubMed record.
Abstract
Modified nucleosides in tRNAs are critical for protein translation. N1-methylguanosine-37 and N1-methylinosine-37 in tRNAs, both located at the 3'-adjacent to the anticodon, are formed by Trm5. Here we describe Arabidopsis thaliana AtTRM5 (At3g56120) as a Trm5 ortholog. Attrm5 mutant plants have overall slower growth as observed by slower leaf initiation rate, delayed flowering and reduced primary root length. In Attrm5 mutants, mRNAs of flowering time genes are less abundant and correlated with delayed flowering. We show that AtTRM5 complements the yeast trm5 mutant, and in vitro methylates tRNA guanosine-37 to produce N1-methylguanosine (m1G). We also show in vitro that AtTRM5 methylates tRNA inosine-37 to produce N1-methylinosine (m1I) and in Attrm5 mutant plants, we show a reduction of both N1-methylguanosine and N1-methylinosine. We also show that AtTRM5 is localized to the nucleus in plant cells. Proteomics data showed that photosynthetic protein abundance is affected in Attrm5 mutant plants. Finally, we show tRNA-Ala aminoacylation is not affected in Attrm5 mutants. However the abundance of tRNA-Ala and tRNA-Asp 5' half cleavage products are deduced. Our findings highlight the bifunctionality of AtTRM5 and the importance of the post-transcriptional tRNA modifications m1G and m1I at tRNA position 37 in general plant growth and development.
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