Evidence map›Paper›PMID 31755214›Full record

ArticleJournal of cellular and molecular medicine2020

Inhibition of cyclin-dependent kinase 7 down-regulates yes-associated protein expression in mesothelioma cells.

Jinbai Miao, Hiroyuki Kyoyama, Luwei Liu, Geraldine Chan, Yucheng Wang, Anatoly Urisman, Yi-Lin Yang, Shu Liu, Zhidong Xu, Hu Bin and 3 more

Open access · goldAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Journal of cellular and molecular medicine, 2020. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 6 papers.

0numbers the graph read from it
0cells of the map it votes in
6citing papers in PubMed
0.7field-weighted citation impact, top 33% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

6 citing papers in PubMed, 7 citations in OpenAlex.

  1. Review
  2. A New CD10 Antibody Inhibits the Growth of Malignant Mesothelioma.Monoclonal antibodies in immunodiagnosis and immunotherapy · 2021
    Article
  3. Hippo-Independent Regulation of Yki/Yap/Taz: A Non-canonical View.Frontiers in cell and developmental biology · 2021
    Review
  4. Genomics and Functional Genomics of Malignant Pleural Mesothelioma.International journal of molecular sciences · 2020
    Review
  5. Review
  6. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

13 authors at 2 institutions in 2 countries.

Jinbai MiaoDepartment of Surgery, Thoracic Oncology Laboratory, Comprehensive Cancer Center, University of California, San Francisco, CA, USA.ORCID 0000-0001-5027-813X
Hiroyuki KyoyamaDepartment of Surgery, Thoracic Oncology Laboratory, Comprehensive Cancer Center, University of California, San Francisco, CA, USA.
Luwei LiuDepartment of Surgery, Thoracic Oncology Laboratory, Comprehensive Cancer Center, University of California, San Francisco, CA, USA.
Geraldine ChanDepartment of Surgery, Thoracic Oncology Laboratory, Comprehensive Cancer Center, University of California, San Francisco, CA, USA.
Yucheng WangDepartment of Surgery, Thoracic Oncology Laboratory, Comprehensive Cancer Center, University of California, San Francisco, CA, USA.
Anatoly UrismanDepartment of Pathology, University of California, San Francisco, CA, USA.
Yi-Lin YangDepartment of Surgery, Thoracic Oncology Laboratory, Comprehensive Cancer Center, University of California, San Francisco, CA, USA.
Shu LiuDepartment of Surgery, Thoracic Oncology Laboratory, Comprehensive Cancer Center, University of California, San Francisco, CA, USA.
Zhidong XuDepartment of Surgery, Thoracic Oncology Laboratory, Comprehensive Cancer Center, University of California, San Francisco, CA, USA.
Hu BinDepartment of Thoracic Surgery, Beijing Chao-Yang Hospital, Affiliated with Capital Medical University, Beijing, China.
Hui LiDepartment of Thoracic Surgery, Beijing Chao-Yang Hospital, Affiliated with Capital Medical University, Beijing, China.
David M JablonsDepartment of Surgery, Thoracic Oncology Laboratory, Comprehensive Cancer Center, University of California, San Francisco, CA, USA.
Liang YouDepartment of Surgery, Thoracic Oncology Laboratory, Comprehensive Cancer Center, University of California, San Francisco, CA, USA.
University of California, San Francisco · USCapital Medical University · CN

Funding

The Role of Cul4A in Genome Stability and Cancer DevelopmentR01CA140654 · NCI · UNIVERSITY OF CALIFORNIA, SAN FRANCISCO · PI YOU, LIANG · 2010 to 2014
$1.5M
NCI NIH HHS R01 CA140654
6 · The paper itself

Abstract

Cyclin-dependent kinase 7 (CDK7) is a protein kinase that plays a major role in transcription initiation. Yes-associated protein (YAP) is a main effector of the Hippo/YAP signalling pathway. Here, we investigated the role of CDK7 on YAP regulation in human malignant pleural mesothelioma (MPM). We found that in microarray samples of human MPM tissue, immunohistochemistry staining showed correlation between the expression level of CDK7 and YAP (n = 70, r = .513). In MPM cells, CDK7 expression level was significantly correlated with GTIIC reporter activity (r = .886, P = .019). Inhibition of CDK7 by siRNA decreased the YAP protein level and the GTIIC reporter activity in the MPM cell lines 211H, H290 and H2052. Degradation of the YAP protein was accelerated after CDK7 knockdown in 211H, H290 and H2052 cells. Inhibition of CDK7 reduced tumour cell migration and invasion, as well as tumorsphere formation ability. Restoration of the CDK7 gene rescued the YAP protein level and GTIIC reporter activity after siRNA knockdown in 211H and H2052 cells. Finally, we performed a co-immunoprecipitation analysis using an anti-YAP antibody and captured the CDK7 protein in 211H cells. Our results suggest that CDK7 inhibition reduces the YAP protein level by promoting its degradation and suppresses the migration and invasion of MPM cells. Cyclin-dependent kinase 7 may be a promising therapeutic target for MPM.

Indexed as

Gene Expression Regulation, NeoplasticAdaptor Proteins, Signal TransducingApoptosisBiomarkers, TumorCase-Control StudiesCell ProliferationCyclin-Dependent Kinase-Activating KinaseCyclin-Dependent KinasesDown-RegulationHumansMesotheliomaPleural NeoplasmsPrognosisSignal TransductionTranscription FactorsTumor Cells, CulturedAdaptor Proteins, Signal TransducingBiomarkers, TumorCDK7 protein, humanCyclin-Dependent Kinase-Activating KinaseCyclin-Dependent KinasesTranscription FactorsYAP1 protein, humanYAP-Signaling Proteinscyclin-dependent kinase 7Hippo pathwaymalignant pleural mesotheliomayes-associated protein

Identifiers

PMID31755214
PMCPMC6933402
OpenAlexW2991378561

What OpenQuestion holds

Textmetadata
LicenceCC BY
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.