ArticleNPJ science of food2019
Food authentication from shotgun sequencing reads with an application on high protein powders.
Article in NPJ science of food, 2019. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 18 papers.
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Who cites it
18 citing papers in PubMed, 44 citations in OpenAlex.
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- Benchmarking and Validation of a Bioinformatics Workflow for Meat Species Identification Using 16S rDNA Metabarcoding.Foods (Basel, Switzerland) · 2023Article
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- The Application of Metagenomics to Study Microbial Communities and Develop Desirable Traits in Fermented Foods.Foods (Basel, Switzerland) · 2022Review
- Using deep learning to detect digitally encoded DNA trigger for Trojan malware in Bio-Cyber attacks.Scientific reports · 2022Article
- Strategies for molecular authentication of herbal products: from experimental design to data analysis.Chinese medicine · 2022Review
- Malnourishment affects gene expression along the length of the small intestine.Frontiers in nutrition · 2022Article
- Metagenomics: A viable tool for reconstructing herbivore diet.Molecular ecology resources · 2021Article
- Molecular Approaches to Agri-Food Traceability and Authentication: An Updated Review.Foods (Basel, Switzerland) · 2021Review
- DNA Extraction and Host Depletion Methods Significantly Impact and Potentially Bias Bacterial Detection in a Biological Fluid.mSystems · 2021Article
- DNA Metabarcoding for Quality Control of Basil, Oregano, and Paprika.Frontiers in plant science · 2021Article
- The Species Identification in Traditional Herbal Patent Medicine, Wuhu San, Based on Shotgun Metabarcoding.Frontiers in pharmacology · 2021Article
- Genomic characterization of a diazotrophic microbiota associated with maize aerial root mucilage.PloS one · 2020Article
Corrections and comments
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Authors and funding
19 authors at 6 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Here we propose that using shotgun sequencing to examine food leads to accurate authentication of ingredients and detection of contaminants. To demonstrate this, we developed a bioinformatic pipeline, FASER (Food Authentication from SEquencing Reads), designed to resolve the relative composition of mixtures of eukaryotic species using RNA or DNA sequencing. Our comprehensive database includes >6000 plants and animals that may be present in food. FASER accurately identified eukaryotic species with 0.4% median absolute difference between observed and expected proportions on sequence data from various sources including sausage meat, plants, and fish. FASER was applied to 31 high protein powder raw factory ingredient total RNA samples. The samples mostly contained the expected source ingredient, chicken, while three samples unexpectedly contained pork and beef. Our results demonstrate that DNA/RNA sequencing of food ingredients, combined with a robust analysis, can be used to find contaminants and authenticate food ingredients in a single assay.
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Registered trials
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