ArticleScientific reports2019
Breakthrough in Marine Invertebrate Cell Culture: Sponge Cells Divide Rapidly in Improved Nutrient Medium.
Article in Scientific reports, 2019. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 21 papers.
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Who cites it
21 citing papers in PubMed, 47 citations in OpenAlex.
- Coating materials enhance urochordate primary cell culture adherence.In vitro cellular & developmental biology. Animal · 2026Article
- The Chemistry and Pharmacology of the Alkaloid Barettin and Its Analogues from the Marine SpongeMarine drugs · 2026Review
- Challenges in cellular agriculture: lessons from Pacific white shrimp, Litopenaeus vannamei.In vitro cellular & developmental biology. Animal · 2025Article
- Advancing marine invertebrate cell line research: four key knowledge gaps.In vitro cellular & developmental biology. Animal · 2025Review
- The chromosomal genome sequence of the giant barrel sponge,Wellcome open research · 2025Article
- The Buds of Oscarella lobularis (Porifera, Homoscleromorpha): A New Convenient Model for Sponge Cell and Evolutionary Developmental Biology.Journal of experimental zoology. Part B, Molecular and developmental evolution · 2024Article
- Whole genome sequence of the deep-sea sponge Geodia barretti (Metazoa, Porifera, Demospongiae).G3 (Bethesda, Md.) · 2023Article
- Improved Media Formulations for Primary Cell Cultures Derived from a Colonial Urochordate.Cells · 2023Article
- First continuous marine sponge cell line established.Scientific reports · 2023Article
- Extracellularly Detectable Electrochemical Signals of Living Cells Originate from Metabolic Reactions.Advanced science (Weinheim, Baden-Wurttemberg, Germany) · 2023Article
- From friends to foes: fungi could be emerging marine sponge pathogens under global change scenarios.Frontiers in microbiology · 2023Article
- Gene delivery available in molluscan cells by strong promoter discovered from bivalve-infectious virus.Proceedings of the National Academy of Sciences of the United States of America · 2022Article
- Biomaterials and Bioactive Natural Products from Marine Invertebrates: From Basic Research to Innovative Applications.Marine drugs · 2022Review
- Development of an in vitro tissue culture system for hammer coral (Fimbriaphyllia ancora) ovaries.Scientific reports · 2021Article
- On the Path to Thermo-Stable Collagen: Culturing the Versatile SpongeMarine drugs · 2021Article
- 3-D Culture of Marine Sponge Cells for Production of Bioactive Compounds.Marine drugs · 2021Article
- Establishing Sustainable Cell Lines of a Coral, Acropora tenuis.Marine biotechnology (New York, N.Y.) · 2021Article
- Cultivation of fractionated cells from a bioactive-alkaloid-bearing marine sponge Axinella sp.In vitro cellular & developmental biology. Animal · 2021Article
- Stem Cells and Innate Immunity in Aquatic Invertebrates: Bridging Two Seemingly Disparate Disciplines for New Discoveries in Biology.Frontiers in immunology · 2021Review
- The Effects of Sampling and Storage Conditions on the Metabolite Profile of the Marine SpongeFrontiers in chemistry · 2021Article
Corrections and comments
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Authors and funding
8 authors at 3 institutions in 3 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Sponges (Phylum Porifera) are among the oldest Metazoa and considered critical to understanding animal evolution and development. They are also the most prolific source of marine-derived chemicals with pharmaceutical relevance. Cell lines are important tools for research in many disciplines, and have been established for many organisms, including freshwater and terrestrial invertebrates. Despite many efforts over multiple decades, there are still no cell lines for marine invertebrates. In this study, we report a breakthrough: we demonstrate that an amino acid-optimized nutrient medium stimulates rapid cell division in 9 sponge species. The fastest dividing cells doubled in less than 1 hour. Cultures of 3 species were subcultured from 3 to 5 times, with an average of 5.99 population doublings after subculturing, and a lifespan from 21 to 35 days. Our results form the basis for developing marine invertebrate cell models to better understand early animal evolution, determine the role of secondary metabolites, and predict the impact of climate change to coral reef community ecology. Furthermore, sponge cell lines can be used to scale-up production of sponge-derived chemicals for clinical trials and develop new drugs to combat cancer and other diseases.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.