Evidence map›Paper›PMID 31754076›Full record

ArticleRNA (New York, N.Y.)2020

A convenient strategy to clone small RNA and mRNA for high-throughput sequencing.

Lichao Li, Hui Dai, An-Phong Nguyen, Weifeng Gu

Open access · bronzeAbstract read
In one paragraph

Article in RNA (New York, N.Y.), 2020. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 16 papers.

0numbers the graph read from it
0cells of the map it votes in
16citing papers in PubMed
1.1field-weighted citation impact, top 22% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

16 citing papers in PubMed, 23 citations in OpenAlex.

  1. Article
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  3. Review
  4. Article
  5. Article
  6. CSDE1 promotes miR-451 biogenesis.Nucleic acids research · 2023
    Article
  7. Analysis of C. elegans Germline Small RNA Pathways.Methods in molecular biology (Clifton, N.J.) · 2023
    Article
  8. Article
  9. Article
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  13. Article
  14. Review
  15. Article
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4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

4 authors at 1 institution in 1 country.

Lichao LiDepartment of Molecular, Cell, and Systems Biology, University of California, Riverside, California 92521, USA.
Hui DaiDepartment of Molecular, Cell, and Systems Biology, University of California, Riverside, California 92521, USA.
An-Phong NguyenDepartment of Molecular, Cell, and Systems Biology, University of California, Riverside, California 92521, USA.
Weifeng GuDepartment of Molecular, Cell, and Systems Biology, University of California, Riverside, California 92521, USA.
University of California, Riverside · US

Funding

A Novel RNA Polyphosphatase PIR-1 Plays Important Roles in Silencing Viruses and Regulating Genes in C. elegansR01GM124349 · NIGMS · UNIVERSITY OF CALIFORNIA RIVERSIDE · PI GU, WEIFENG · 2018 to 2022
$1.8M
NIGMS NIH HHS R01 GM124349
6 · The paper itself

Abstract

High-throughput sequencing has become a standard tool for analyzing RNA and DNA. This method usually needs a cDNA/DNA library ligated with specific 5' and 3' linkers. Unlike mRNA, small RNA often contains modifications including 5' cap or triphosphate and 2'-

Indexed as

Cloning, MolecularHigh-Throughput Nucleotide SequencingAnimalsCaenorhabditis elegansDNA, ComplementaryDNA PrimersGene LibraryPolymerase Chain ReactionRNA, HelminthRNA, MessengerSensitivity and SpecificitySequence Analysis, RNADNA, ComplementaryDNA PrimersRNA, HelminthRNA, Messengerconstructing sequencing libraries without gel purificationhigh-throughput or next-generation sequencingmRNA cloningRNA modificationssmall RNA cloning

Identifiers

PMID31754076
PMCPMC6961543
OpenAlexW2991376477

What OpenQuestion holds

Textmetadata
LicenceCC BY
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.