ArticleScience (New York, N.Y.)2019
Computational design of a modular protein sense-response system.
Article in Science (New York, N.Y.), 2019. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 65 papers.
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Who cites it
65 citing papers in PubMed.
- Genetically encoded tools for tracking metabolites in live cells.Biochemical Society transactions · 2026Review
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- De novo design of a macrocycle-induced dimerization system for cellular control.Nature communications · 2026Article
- De novo design of a macrocycle induced dimerization system for cellular control.bioRxiv : the preprint server for biology · 2026Article
- A Hybrid Physics-Deep Learning Framework for Combinatorial De Novo Design of Small-Molecule Binding Proteins.bioRxiv : the preprint server for biology · 2026Article
- Review
- Small-molecule binding and sensing with a designed protein family.Nature communications · 2026Article
- Real-Time Tracking of Intracellular Prenyl Phosphate Pools in the Marine DiatomACS synthetic biology · 2026Article
- De novo protein design: a transformative frontier in clinical protein applications.Journal of translational medicine · 2026Review
- Repurposing nuclear receptors for ligand-responsive liquid condensate formation and gene regulation.Nature communications · 2026Article
- MGTbind: a comprehensive database of molecular glue ternary interactome.Nucleic acids research · 2026Article
- Unusually broad-spectrum small-molecule sensing using a single protein scaffold.Proceedings of the National Academy of Sciences of the United States of America · 2025Article
- Protein A-like peptide generation based on generalized diffusion model.Journal of computer-aided molecular design · 2025Article
- PYR1 Biosensor-Driven Genome-Wide CRISPR Screens for Improved Monoterpene Production inACS synthetic biology · 2025Article
- De Novo Design of High-Performance Cortisol Luminescent Biosensors.Journal of the American Chemical Society · 2025Article
- A Designed ZnJournal of the American Chemical Society · 2025Article
- Ligand-induced assembly of antibody variable fragments for the chemical regulation of biological processes.Cell chemical biology · 2025Article
- Article
- Engineering modular and tunable single-molecule sensors by decoupling sensing from signal output.Nature nanotechnology · 2025Article
- Efficient Generation of Protein Pockets with PocketGen.bioRxiv : the preprint server for biology · 2024Article
5 more citing papers are in PubMed but not listed here.
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Authors and funding
15 authors.
Funding
Abstract
Sensing and responding to signals is a fundamental ability of living systems, but despite substantial progress in the computational design of new protein structures, there is no general approach for engineering arbitrary new protein sensors. Here, we describe a generalizable computational strategy for designing sensor-actuator proteins by building binding sites de novo into heterodimeric protein-protein interfaces and coupling ligand sensing to modular actuation through split reporters. Using this approach, we designed protein sensors that respond to farnesyl pyrophosphate, a metabolic intermediate in the production of valuable compounds. The sensors are functional in vitro and in cells, and the crystal structure of the engineered binding site closely matches the design model. Our computational design strategy opens broad avenues to link biological outputs to new signals.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.