ArticleThe Journal of biological chemistry2019
High-resolution genome-wide expression analysis of single myofibers using SMART-Seq.
Article in The Journal of biological chemistry, 2019. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 22 papers.
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Who cites it
22 citing papers in PubMed.
- Age-related transcriptional drift and physiological adaptation in long-living Ames dwarf skeletal muscle.NAR molecular medicine · 2026Article
- Gene Expression Analysis on Single Myofibers from Mouse Edl and Soleus Muscles.Methods in molecular biology (Clifton, N.J.) · 2026Article
- REST/NRSF Preserves muscle stem cell identity by repressing alternate cell fate.Nature communications · 2025Article
- Integrated single-cell functional-proteomic profiling reveals a shift in myofibre specificity in human nemaline myopathy: A proof-of-principle study.The Journal of physiology · 2025Article
- Single-cell omics: experimental workflow, data analyses and applications.Science China. Life sciences · 2025Review
- Cell2Read: an automated workflow to generate sequencing-ready DNA libraries from human cell suspensions.Biology methods & protocols · 2025Article
- The E3 ubiquitin ligase Nedd4L preserves skeletal muscle stem cell quiescence by inhibiting their activation.iScience · 2024Article
- Epigenetic control of skeletal muscle atrophy.Cellular & molecular biology letters · 2024Review
- Transcriptional reprogramming of skeletal muscle stem cells by the niche environment.Nature communications · 2023Article
- Application of Single-Cell and Spatial Omics in Musculoskeletal Disorder Research.International journal of molecular sciences · 2023Review
- Application of ATAC-Seq for genome-wide analysis of the chromatin state at single myofiber resolution.eLife · 2022Article
- Heterogeneous Skeletal Muscle Cell and Nucleus Populations Identified by Single-Cell and Single-Nucleus Resolution Transcriptome Assays.Frontiers in genetics · 2022Review
- Transcriptional networks controlling stromal cell differentiation.Nature reviews. Molecular cell biology · 2021Review
- Article
- Single cell RNA-seq analysis of the flexor digitorum brevis mouse myofibers.Skeletal muscle · 2021Article
- Single-Cell RNAseq Analysis of lncRNAs.Methods in molecular biology (Clifton, N.J.) · 2021Article
- Myf6/MRF4 is a myogenic niche regulator required for the maintenance of the muscle stem cell pool.EMBO reports · 2020Article
- Degenerative and regenerative pathways underlying Duchenne muscular dystrophy revealed by single-nucleus RNA sequencing.Proceedings of the National Academy of Sciences of the United States of America · 2020Article
- Article
- TRPV4 Antagonism Prevents Mechanically Induced Myotonia.Annals of neurology · 2020Article
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Authors and funding
6 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Skeletal muscle is a heterogeneous tissue. Individual myofibers that make up muscle tissue exhibit variation in their metabolic and contractile properties. Although biochemical and histological assays are available to study myofiber heterogeneity, efficient methods to analyze the whole transcriptome of individual myofibers are lacking. Here, we report on a single-myofiber RNA-sequencing (smfRNA-Seq) approach to analyze the whole transcriptome of individual myofibers by combining single-fiber isolation with Switching Mechanism at 5' end of RNA Template (SMART) technology. Using smfRNA-Seq, we first determined the genes that are expressed in the whole muscle, including in nonmyogenic cells. We also analyzed the differences in the transcriptome of myofibers from young and old mice to validate the effectiveness of this new method. Our results suggest that aging leads to significant changes in the expression of metabolic genes, such as
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