Evidence map›Paper›PMID 31752963›Full record

ArticleArthritis research & therapy2019

B lymphocyte stimulator modulates number and function of endothelial progenitor cells in systemic lupus erythematosus.

Francesca Romana Spinelli, Cristiana Barbati, Fulvia Cecarelli, Francesca Morello, Tania Colasanti, Marta Vomero, Laura Massaro, Valeria Orefice, Cristiano Alessandri, Guido Valesini and 1 more

Open access · goldAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Arthritis research & therapy, 2019. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 13 papers.

0numbers the graph read from it
0cells of the map it votes in
13citing papers in PubMed
1.4field-weighted citation impact, top 18% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

13 citing papers in PubMed, 26 citations in OpenAlex.

  1. Article
  2. Review
  3. Review
  4. Article
  5. Review
  6. Review
  7. Article
  8. Review
  9. Cardiovascular disease in systemic lupus erythematosus.Rheumatology and immunology research · 2021
    Review
  10. Review
  11. Review
  12. Review
  13. Review
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

11 authors at 1 institution in 1 country.

Francesca Romana SpinelliDepartment of Internal Medicine and Medical Specialties, Rheumatology, Sapienza University of Rome, Rome, Italy. francescaromana.spinelli@uniroma1.it.
Cristiana BarbatiDepartment of Internal Medicine and Medical Specialties, Rheumatology, Sapienza University of Rome, Rome, Italy.
Fulvia CecarelliDepartment of Internal Medicine and Medical Specialties, Rheumatology, Sapienza University of Rome, Rome, Italy.
Francesca MorelloDepartment of Internal Medicine and Medical Specialties, Rheumatology, Sapienza University of Rome, Rome, Italy.
Tania ColasantiDepartment of Internal Medicine and Medical Specialties, Rheumatology, Sapienza University of Rome, Rome, Italy.
Marta VomeroDepartment of Internal Medicine and Medical Specialties, Rheumatology, Sapienza University of Rome, Rome, Italy.
Laura MassaroDepartment of Internal Medicine and Medical Specialties, Rheumatology, Sapienza University of Rome, Rome, Italy.
Valeria OreficeDepartment of Internal Medicine and Medical Specialties, Rheumatology, Sapienza University of Rome, Rome, Italy.
Cristiano AlessandriDepartment of Internal Medicine and Medical Specialties, Rheumatology, Sapienza University of Rome, Rome, Italy.
Guido ValesiniDepartment of Internal Medicine and Medical Specialties, Rheumatology, Sapienza University of Rome, Rome, Italy.
Fabrizio ContiDepartment of Internal Medicine and Medical Specialties, Rheumatology, Sapienza University of Rome, Rome, Italy.
Sapienza University of Rome · IT

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

backgroundCirculating endothelial progenitor cells (EPCs) are biologic markers of endothelial function. In patients with systemic lupus erythematosus (SLE), the numerical reduction and functional impairment of EPCs contribute to the endothelial dysfunction. Through ex vivo and in vitro studies, we aimed at evaluating the effects of B lymphocyte stimulator (BLyS) on EPC colonies and endothelial cells and also investigating BLyS receptor expression on these cells.

methodsEPCs were isolated from peripheral blood mononuclear cells (PBMC). In order to evaluate their ability to form colonies, EPCs were cultured on fibronectin-coated dishes and incubated with BlyS alone or BlyS and belimumab. Apoptosis of EPCs and endothelial cell line EA.hy926 was evaluated after 6, 12, and 24 h of incubation with BLyS and after 6 h with BLyS and belimumab. The expression of B cell activating factor-receptor (BAFF-R), B cell maturation antigen (BCMA), and transmembrane activator and calcium modulator and cyclophilin ligand (CAML) interactor (TACI) on EPCs and EA.hy926 was analyzed by cytofluorimetry.

resultsThe number of EPC colonies was lower in patients than in controls. Moreover, the colonies from SLE patients were poorly organized compared to controls; the addition of belimumab restored the colony structure. Incubation with BLyS induced apoptosis of EPCs and EA.hy926 that was inhibited by the co-incubation with belimumab. BAFF-R and BCMA were expressed on both EPCs and EA.hy926, while TACI was expressed only on EPCs.

conclusionsEPCs and endothelial cells preferentially express BAFF-R which could be involved in the pro-apoptotic effect of BlyS. Belimumab administration seems to restore the quantitative and qualitative changes of EPC colonies both ex vivo and in vitro.

Indexed as

AdultAntibodies, Monoclonal, HumanizedApoptosisB-Cell Activating FactorB-Cell Activation Factor ReceptorB-Cell Maturation AntigenCell CountCell LineCells, CulturedEndothelial Progenitor CellsFemaleHumansImmunosuppressive AgentsLupus Erythematosus, SystemicMiddle AgedStem CellsAntibodies, Monoclonal, HumanizedB-Cell Activating FactorB-Cell Activation Factor ReceptorB-Cell Maturation AntigenbelimumabImmunosuppressive AgentsTNFRSF13C protein, humanTNFRSF17 protein, humanTransmembrane Activator and CAML Interactor ProteinAtherosclerosisBelimumabBLySEndothelial progenitor cellsSystemic lupus erythematosus

Identifiers

PMID31752963
PMCPMC6868730
OpenAlexW2990930920

What OpenQuestion holds

Textmetadata
LicenceCC BY
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.