ArticleBMC veterinary research2019
Comparison of four commercially available ELISA kits for diagnosis of Fasciola hepatica in Irish cattle.
Article in BMC veterinary research, 2019. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 12 papers.
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Who cites it
12 citing papers in PubMed, 32 citations in OpenAlex.
- Fasciola hepatica in alpine dairy farming: prevalence trends, risk factors and associations with Salmonella Dublin seropositivity.BMC veterinary research · 2026Article
- Spread and dynamics of Calicophoron daubneyi and Fasciola hepatica in an extensively kept water buffalo herd and suitability of an ELISA for detecting antibodies against F. hepatica.Parasitology research · 2026Article
- Advancement in Diagnosis, Treatment, and Vaccines againstPathogens (Basel, Switzerland) · 2024Review
- Diagnosis of fasciolosis antibodies in Brazilian cattle through ELISA employing both native and recombinant antigens.Microbiology spectrum · 2024Article
- The Detection of Circulating Antigen Glutathione S-Transferase in Sheep Infected withAnimals : an open access journal from MDPI · 2024Article
- Immunol detection of cathepsin L fromActa biochimica et biophysica Sinica · 2023Article
- Immunodiagnosis of Fascioliasis in Ruminants by ELISA Method: A Mini-Review.The Malaysian journal of medical sciences : MJMS · 2023Review
- A survey for antibodies against Fasciola hepatica in cattle and sheep in finland indicates a low level of exposure.Acta veterinaria Scandinavica · 2023Article
- Fasciolosis-An Increasing Challenge in the Sheep Industry.Animals : an open access journal from MDPI · 2022Review
- Occurrence and Distribution of Fasciolosis in a Cohort of Ovine Livestock Restricted to a Mountain Plateau in Central Portugal.Animals : an open access journal from MDPI · 2021Article
- A contemporary map of Fasciola hepatica distribution in sheep and cattle in New South Wales.Australian veterinary journalArticle
- The global prevalence of human fascioliasis: a systematic review and meta-analysis.Therapeutic advances in infectious diseaseArticle
Corrections and comments
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Authors and funding
8 authors at 3 institutions in 1 country.
Funding
Abstract
backgroundFasciola hepatica is a liver parasite of mammals and it results in poor welfare outcomes and economic losses in ruminants. While faecal egg count is the test most commonly used for diagnosis, it does not indicate presence of migrating immature stages. Serological techniques increase sensitivity at all stages of the liver fluke infection. The aim of this study was to compare four commercially available ELISA tests for the diagnosis of F. hepatica. For this purpose, we tested three sample types; (i) known F. hepatica status sera from an experimental infection for the comparison of sensitivities and specificities, (ii) sera from pre- and post-flukicide-treated (albendazole, closantel, nitroxynil and triclabendazole) beef cattle to contrast the differences of seropositivity before and after treatment, and (iii) bulk tank milk samples from dairy herds sampled during high and low F. hepatica exposure periods for assessing seasonal variations with the four tests available. Samples were tested using ELISA kits supplied by four manufacturers (Ildana Biotech, IDEXX, Svanova, and Bio-X). Samples were analysed simultaneously and in duplicate.
resultsIn the control population Ildana, IDEXX and Bio-X presented 100% sensitivity (Se) and specificity (Sp), Svanovir presented a Se of 59% and a Sp of 96%. In flukicide-treated beef cattle, kits highlighted decreasing antibody levels 90 days post-treatment in variable degrees. Finally, bulk milk showed a significant decrease in ELISA value between high and low fluke exposure periods with all tests studied.
conclusionsSe and Sp found in the present study, confirm that Ildana, IDEXX and Bio-X are accurate for the detection of F. hepatica exposure in Irish cattle. Svanovir Se and Sp in this population, indicate that a larger study is necessary to confirm this test characteristic in Irish herds. In post-treatment use, Bio-X showed a consistent and significant decrease of ELISA value in all groups treated, denoting to be a reliable tool for assessing treatment effect at 90 days post-treatment. Finally, all tests showed to be a reliable tool for the F. hepatica monitoring of high and low exposure seasons, using bulk tank milk samples.
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