Evidence map›Paper›PMID 31752416›Full record

ArticleGenes2019

Analysis of Soybean Somatic Embryogenesis Using Chromosome Segment Substitution Lines and Transcriptome Sequencing.

Si-Nan Li, Peng Cheng, Yun-Qi Bai, Yan Shi, Jing-Yao Yu, Rui-Chao Li, Run-Nan Zhou, Zhan-Guo Zhang, Xiao-Xia Wu, Qing-Shan Chen

Abstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Genes, 2019. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 2 papers.

0numbers the graph read from it
0cells of the map it votes in
2citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

2 citing papers in PubMed.

  1. Article
  2. Frontiers in plant science · 2025
    Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

10 authors.

Si-Nan LiCollege of Agriculture, Northeast Agricultural University, Harbin 150030, Heilongjiang, China.
Peng ChengCollege of Agriculture, Northeast Agricultural University, Harbin 150030, Heilongjiang, China.
Yun-Qi BaiCollege of Agriculture, Northeast Agricultural University, Harbin 150030, Heilongjiang, China.
Yan ShiCollege of Agriculture, Northeast Agricultural University, Harbin 150030, Heilongjiang, China.
Jing-Yao YuCollege of Agriculture, Northeast Agricultural University, Harbin 150030, Heilongjiang, China.ORCID 0000-0001-7024-9096
Rui-Chao LiCollege of Agriculture, Northeast Agricultural University, Harbin 150030, Heilongjiang, China.
Run-Nan ZhouCollege of Agriculture, Northeast Agricultural University, Harbin 150030, Heilongjiang, China.
Zhan-Guo ZhangCollege of Agriculture, Northeast Agricultural University, Harbin 150030, Heilongjiang, China.
Xiao-Xia WuCollege of Agriculture, Northeast Agricultural University, Harbin 150030, Heilongjiang, China.
Qing-Shan ChenCollege of Agriculture, Northeast Agricultural University, Harbin 150030, Heilongjiang, China.

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

Soybean is an important cash crop that is widely used as a source of vegetable protein and edible oil. The regeneration ability of soybean directly affects the application of biotechnology. In this study, we used the exogenous hormone 2,4-D to treat immature embryos. Different levels of somatic incidence were selected from the chromosome segment substitution lines (CSSLs) constructed by SN14 and ZYD00006. Transcriptome sequencing of extreme materials was performed, and 2666 differentially expressed genes were obtained. At the same time, a difference table was generated by combining the data on CSSL rearrangement. In the extreme materials, a total of 93 differentially expressed genes were predicted and were then analyzed by cluster analysis and Gene Ontology (GO) annotation. After screening and annotating the target genes, three differentially expressed genes with hormone pathways were identified. The expression patterns of the target genes were verified by real-time quantitative PCR (qRT-PCR). Haplotype polymorphism detection and linkage disequilibrium analysis were performed on the candidate gene

Indexed as

Plant Somatic Embryogenesis Techniques2,4-Dichlorophenoxyacetic AcidBiotechnologyChromosomes, PlantCluster AnalysisCrops, AgriculturalExome SequencingGene Expression ProfilingGene Expression Regulation, PlantGene OntologyGlycine maxMolecular Sequence AnnotationPlants, Genetically ModifiedRegenerationSeedsTranscriptome2,4-Dichlorophenoxyacetic AcidCSSLhaplotype analysisRT-qPCRsomatic embryogenesissoybeantranscriptome sequencing

Identifiers

PMID31752416
PMCPMC6896167

What OpenQuestion holds

Textmetadata
LicenceCC BY
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.