Evidence map›Paper›PMID 31752401›Full record

ArticleMolecules (Basel, Switzerland)2019

Determination of Lactoferrin in Camel Milk by Ultrahigh-Performance Liquid Chromatography-Tandem Mass Spectrometry Using an Isotope-Labeled Winged Peptide as Internal Standard.

Xia Li, Zengmei Li, Enmin Xu, Ling Chen, Hua Feng, Lu Chen, Ligang Deng, Dongliang Guo

Open access · goldAbstract read
In one paragraph

Article in Molecules (Basel, Switzerland), 2019. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 7 papers.

0numbers the graph read from it
0cells of the map it votes in
7citing papers in PubMed
2.0field-weighted citation impact, top 14% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

7 citing papers in PubMed, 25 citations in OpenAlex.

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4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

8 authors at 1 institution in 1 country.

Xia LiInstitute of Agricultural Quality Standards and Testing Technology Research, Shandong Academy of Agricultural Sciences, 202 Gongyebeilu Road, Jinan 250100, Shandong Province, China.
Zengmei LiInstitute of Agricultural Quality Standards and Testing Technology Research, Shandong Academy of Agricultural Sciences, 202 Gongyebeilu Road, Jinan 250100, Shandong Province, China.
Enmin XuShandong Veterinary Drug Quality Inspection Institute, 68 Huaicun Street, Jinan, Shandong Province, China.
Ling ChenShandong Veterinary Drug Quality Inspection Institute, 68 Huaicun Street, Jinan, Shandong Province, China.
Hua FengInstitute of Agricultural Quality Standards and Testing Technology Research, Shandong Academy of Agricultural Sciences, 202 Gongyebeilu Road, Jinan 250100, Shandong Province, China.
Lu ChenInstitute of Agricultural Quality Standards and Testing Technology Research, Shandong Academy of Agricultural Sciences, 202 Gongyebeilu Road, Jinan 250100, Shandong Province, China.
Ligang DengInstitute of Agricultural Quality Standards and Testing Technology Research, Shandong Academy of Agricultural Sciences, 202 Gongyebeilu Road, Jinan 250100, Shandong Province, China.
Dongliang GuoInstitute of Agricultural Quality Standards and Testing Technology Research, Shandong Academy of Agricultural Sciences, 202 Gongyebeilu Road, Jinan 250100, Shandong Province, China.
Shandong Academy of Agricultural Sciences · CN

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

An ultrahigh-performance liquid chromatography-tandem mass spectrometry method was developed and validated for the determination of lactoferrin in camel milk based on the signature peptide. The camel lactoferrin was purified by heparin affinity chromatography and then used to screen tryptic signature peptides. The signature peptide was selected on the basis of sequence database search and identified from the tryptic hydrolysates of purified camel lactoferrin by ultrahigh-performance liquid chromatography and quadrupole time-of-flight tandem mass spectrometry. The pretreatment procedures included the addition of isotope-labeled winged peptide and the disposal of lipids and caseins followed by an enzymatic digestion with trypsin. Analytes were separated on an Acquity UPLC BEH 300 C18 column and then detected on a triple-quadrupole mass spectrometer in 7 min. The limits of detection and quantification were 3.8 mg kg

Indexed as

Chromatography, High Pressure LiquidIsotope LabelingTandem Mass SpectrometryAnimalsCamelusFood AnalysisLactoferrinMilkPeptidesReference StandardsReproducibility of ResultsSensitivity and SpecificityLactoferrinPeptidescamel milkheparin affinity chromatographylactoferrinultrahigh-performance liquid chromatography-tandem mass spectrometry

Identifiers

PMID31752401
PMCPMC6891602
OpenAlexW2989866258

What OpenQuestion holds

Textmetadata
LicenceCC BY
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.