ArticlePloS one2019
Effect of repeated in vivo microCT imaging on the properties of the mouse tibia.
Article in PloS one, 2019. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 18 papers.
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Who cites it
18 citing papers in PubMed, 28 citations in OpenAlex.
- No evidence of xylem embolism refilling during recovery from drought stress in intact sunflowers.Journal of experimental botany · 2026Article
- Quantifying the local strain energy density distribution in the mouse tibia: the critical role of the loading direction.Biomechanics and modeling in mechanobiology · 2025Article
- Xylem embolism refilling revealed in stems of a weedy grass.Proceedings of the National Academy of Sciences of the United States of America · 2025Article
- A robust deep learning approach for segmenting cortical and trabecular bone from 3D high resolution µCT scans of mouse bone.Scientific reports · 2025Article
- Cortical bone adaptation response is region specific, but not peak load dependent: insights fromBiomechanics and modeling in mechanobiology · 2024Article
- A novel framework for elucidating the effect of mechanical loading on the geometry of ovariectomized mouse tibiae using principal component analysis.Frontiers in bioengineering and biotechnology · 2024Article
- The loading direction dramatically affects the mechanical properties of the mouse tibia.Frontiers in bioengineering and biotechnology · 2024Article
- Osteoporosis and Covid-19: Detected similarities in bone lacunar-level alterations via combined AI and advanced synchrotron testing.Materials & design · 2023Article
- Direct Assessment of Rabbit Cortical Bone Basic Multicellular Unit Longitudinal Erosion Rate: A 4D Synchrotron-Based Approach.Journal of bone and mineral research : the official journal of the American Society for Bone and Mineral Research · 2022Article
- Accuracy ofFrontiers in endocrinology · 2022Article
- Influence of X-rays and gamma-rays on the mechanical performance of human bone factoring out intraindividual bone structure and composition indices.Materials today. Bio · 2022Article
- Identification of Small-Molecule Inhibitors for Osteosarcoma Targeted Therapy: SynchronizingFrontiers in bioengineering and biotechnology · 2022Article
- Reproducibility of Densitometric and Biomechanical Assessment of the Mouse Tibia FromFrontiers in endocrinology · 2022Article
- Non-invasive prediction of the mouse tibia mechanical properties from microCT images: comparison between different finite element models.Biomechanics and modeling in mechanobiology · 2021Article
- A Review on Multiscale Bone Damage: From the Clinical to the Research Perspective.Materials (Basel, Switzerland) · 2021Review
- Bone Phenotyping Approaches in Human, Mice and Zebrafish - Expert Overview of the EU Cost Action GEMSTONE ("GEnomics of MusculoSkeletal traits TranslatiOnal NEtwork").Frontiers in endocrinology · 2021Review
- The Role of the Loading Condition in Predictions of Bone Adaptation in a Mouse Tibial Loading Model.Frontiers in bioengineering and biotechnology · 2021Article
- PTH(1-34) treatment and/or mechanical loading have different osteogenic effects on the trabecular and cortical bone in the ovariectomized C57BL/6 mouse.Scientific reports · 2020Article
Corrections and comments
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Authors and funding
4 authors at 2 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
In longitudinal studies, in vivo micro-Computed Tomography (microCT) imaging is used to investigate bone changes over time due to interventions in mice. However, ionising radiation can provoke significant variations in bone morphometric parameters. In a previous study, we evaluated the effect of reducing the integration time on the properties of the mouse tibia measured from microCT images. A scanning procedure (100 ms integration time, 256 mGy nominal radiation dose) was selected as the best compromise between image quality and radiation dose induced on the animal. In this work, the effect of repeated in vivo scans has been evaluated using the selected procedure. The right tibia of twelve female C57BL/6 (six wild type, WT, six ovariectomised, OVX) and twelve BALB/c (six WT, six OVX) mice was scanned every two weeks, starting at week 14 of age. At week 24, mice were sacrificed and both tibiae were scanned. Standard trabecular and cortical morphometric parameters were calculated. The spatial distribution of densitometric parameters (e.g. bone mineral content) was obtained by dividing each tibia in 40 partitions. Stiffness and strength in compression were estimated using homogeneous linear elastic microCT-based micro-Finite Element models. Differences between right (irradiated) and left (non-irradiated control) tibiae were evaluated for each parameter. The irradiated tibiae had higher Tb.Th (+3.3%) and Tb.Sp (+11.6%), and lower Tb.N (-14.2%) compared to non-irradiated tibiae, consistently across both strains and intervention groups. A reduction in Tb.BV/TV (-14.9%) was also observed in the C57BL/6 strain. In the OVX group, a small reduction was also observed in Tt.Ar (-5.0%). In conclusion, repeated microCT scans (at 256 mGy, 5 scans, every two weeks) had limited effects on the mouse tibia, compared to the expected changes induced by bone treatments. Therefore, the selected scanning protocol is acceptable for measuring the effect of bone interventions in vivo.
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