ArticleNucleic acids research2019
A tight cold-inducible switch built by coupling thermosensitive transcriptional and proteolytic regulatory parts.
Article in Nucleic acids research, 2019. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 14 papers.
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Who cites it
14 citing papers in PubMed, 44 citations in OpenAlex.
- De novo designed protein guiding targeted protein degradation.Nature communications · 2025Article
- LowTempGAL: a highly responsive low temperature-inducible GAL system in Saccharomyces cerevisiae.Nucleic acids research · 2024Article
- Accelerating Genetic Sensor Development, Scale-up, and Deployment Using Synthetic Biology.Biodesign research · 2024Article
- RelCurator: a text mining-based curation system for extracting gene-phenotype relationships specific to neurodegenerative disorders.Genes & genomics · 2023Article
- A dual-regulation inducible switch system for microRNA detection and cell type-specific gene activation.Theranostics · 2023Article
- Recent Synthetic Biology Approaches for Temperature- and Light-Controlled Gene Expression in Bacterial Hosts.Molecules (Basel, Switzerland) · 2022Review
- Tunable Temperature-Sensitive Transcriptional Activation Based on Lambda Repressor.ACS synthetic biology · 2022Article
- Exploiting synthetic regulatory elements for non-dominant microorganisms.Synthetic and systems biotechnology · 2022Article
- Robust network topologies for temperature-inducible bioswitches.Journal of biological engineering · 2022Article
- Microbial Biocontainment Systems for Clinical, Agricultural, and Industrial Applications.Frontiers in bioengineering and biotechnology · 2022Review
- Cybergenetic control of microbial community composition.Frontiers in bioengineering and biotechnology · 2022Review
- Dynamic control in metabolic engineering: Theories, tools, and applications.Metabolic engineering · 2021Review
- Atypical chemokine receptor ACKR2-V41A has decreased CCL2 binding, scavenging, and activation, supporting sustained inflammation and increased Alzheimer's disease risk.Scientific reports · 2020Article
- Translating New Synthetic Biology Advances for Biosensing Into the Earth and Environmental Sciences.Frontiers in microbiology · 2020Review
Corrections and comments
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Authors and funding
9 authors at 3 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Natural organisms have evolved intricate regulatory mechanisms that sense and respond to fluctuating environmental temperatures in a heat- or cold-inducible fashion. Unlike dominant heat-inducible switches, very few cold-inducible genetic switches are available in either natural or engineered systems. Moreover, the available cold-inducible switches still have many shortcomings, including high leaky gene expression, small dynamic range (<10-fold) or broad transition temperature (>10°C). To address these problems, a high-performance cold-inducible switch that can tightly control target gene expression is highly desired. Here, we introduce a tight and fast cold-inducible switch that couples two evolved thermosensitive variants, TFts and TEVts, as well as an additional Mycoplasma florum Lon protease (mf-Lon) to effectively turn-off target gene expression via transcriptional and proteolytic mechanisms. We validated the function of the switch in different culture media and various Escherichia coli strains and demonstrated its tightness by regulating two morphogenetic bacterial genes and expressing three heat-unstable recombinant proteins, respectively. Moreover, the additional protease module enabled the cold-inducible switch to actively remove the pre-existing proteins in slow-growing cells. This work establishes a high-performance cold-inducible system for tight and fast control of gene expression which has great potential for basic research, as well as industrial and biomedical applications.
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