Evidence map›Paper›PMID 31749365›Full record

ArticleThe Journal of organic chemistry2020

PEGylation near a Patch of Nonpolar Surface Residues Increases the Conformational Stability of the WW Domain.

Steven R E Draper, Dallin S Ashton, Benjamin M Conover, Anthony J Carter, Kimberlee L Stern, Qiang Xiao, Joshua L Price

Abstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in The Journal of organic chemistry, 2020. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 1 paper.

0numbers the graph read from it
0cells of the map it votes in
1citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

1 citing paper in PubMed.

  1. Review
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

7 authors.

Steven R E DraperDepartment of Chemistry and Biochemistry , Brigham Young University , Provo , Utah 84602 , United States.
Dallin S AshtonDepartment of Chemistry and Biochemistry , Brigham Young University , Provo , Utah 84602 , United States.
Benjamin M ConoverDepartment of Chemistry and Biochemistry , Brigham Young University , Provo , Utah 84602 , United States.
Anthony J CarterDepartment of Chemistry and Biochemistry , Brigham Young University , Provo , Utah 84602 , United States.
Kimberlee L SternDepartment of Chemistry and Biochemistry , Brigham Young University , Provo , Utah 84602 , United States.
Qiang XiaoDepartment of Chemistry and Biochemistry , Brigham Young University , Provo , Utah 84602 , United States.
Joshua L PriceDepartment of Chemistry and Biochemistry , Brigham Young University , Provo , Utah 84602 , United States.ORCID 0000-0002-0116-0968

Funding

Predictive Structure-based Guidelines for Identifying Optimal PEGylation Sites within Proteins and Understanding the Context-Dependence of Non-covalent Interaction StrengthR15GM116055 · NIGMS · BRIGHAM YOUNG UNIVERSITY · PI PRICE, JOSHUA L · 2015 to 2020
$781k
NIGMS NIH HHS R15 GM116055
6 · The paper itself

Abstract

Many proteins have one or more surface-exposed patches of nonpolar residues; our observations here suggest that PEGylation near such locations might be a useful strategy for increasing protein conformational stability. Specifically, we show that conjugating a PEG-azide to a propargyloxyphenylalanine via the copper(I)-catalyzed azide-alkyne cycloaddition can increase the conformational stability of the WW domain due to a favorable synergistic effect that depends on the hydrophobicity of a nearby patch of nonpolar surface residues.

Indexed as

Polyethylene GlycolsProteinsAlkynesAzidesCopperProtein ConformationProtein StabilityWW DomainsAlkynesAzidesCopperPolyethylene GlycolsProteins

Identifiers

PMID31749365
PMCPMC8147659

What OpenQuestion holds

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Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.